Suchergebnisse

The objective was to determine chemical composition and in situ degradability for silages of dual-purpose sorghum hybrids. Twenty-five hybrids of double-purpose sorghum were investigated in a randomized block design with three replicates. Ammoniacal nitrogen (NH3-N), pH, chemical composition of the silage and in situ degradability were determined. Silage of the hybrids 9929012, 947216, 947030, 947254, 947072, 947252, 12F042066, 1141570 and 1141562 presented greater dry matter content, ranging from 402.9 to 470.7 g kg-1. The greatest crude protein content was presented by silages from the hybrids 9929036, 9929030, 12F042224, FEPAGRO19, FEPAGRO11, 9929026, 947030, 947072, 947252, 12F042226 and BRS Ponta Negra. The silage of hybrid 1141562 exhibited the lowest neutral detergent fiber content (588 g kg-1). The highest values of dry matter and crude protein degradability were observed in the silages of the hybrids 9929030, 947252, 947072, 12F042066 and 12F042226. The use of ensilage of the 9929030 and 12F042226 hybrids is recommended because these silages present a better chemical composition of dry matter, crude protein and neutral detergent fiber and better dry matter and crude protein degradability.

Visceral leishmaniasis (VL) is a zoonosis caused by the protozoan of the genus Leishmania. The disease is transmitted by the bite of a sand fly vector. Although the main reservoirs are dogs, other hosts can be infected and may play this role. Rio Grande do Sul western region, located on the triple border of Brazil-Uruguay-Argentina, represents a VL transmission area. The goal of the present study was to identify Leishmania spp. infection in animals from rural areas of Uruguaiana, Rio Grande do Sul. Nine farms in the Uruguaiana municipality, Rio Grande do Sul state, were included. Peripheral blood samples were collected from 113 animals (canine [n=22], equine [n=91]) for detection of Leishmania spp. DNA was isolated and polymerase chain reaction was performed. Eight (7%) animals with Leishmania spp. infection were detected on two farms in the same geographical area, seven of which were horses and one was canine, all of which were asymptomatic. To investigate the species of Leishmania, one of the positive equine samples was subjected to direct sequencing, which confirmed the presence of L. infantum genetic material. Results of this study confirm the presence of L. infantum-infected animals in rural areas of Uruguaiana, and provide evidence supporting further investigation of risk factors for dissemination in such areas.