Suchergebnisse

Das Anliegen dieses Bandes ist es, sexuelle und geschlechtliche Vielfalt stärker ins Bewusstsein pädagogischer Kontexte zu rücken. Die Evaluationsstudie, die dieser Herausgabe voranging, weist auf ein Interesse und eine Bereitschaft hin, das Thema "Sexuelle Vielfalt" in der pädagogischen Praxis aufzugreifen und zu bearbeiten, macht aber zugleich gravierende Schwierigkeiten hierbei deutlich. Die präsentierten Bildungsbausteine und Anwendungsvorschläge sind als Respons auf diesen Befund zu verstehen und einhergehend damit als Hilfestellung für Praktiker_innen gedacht.

8 páginas, 3 figuras, 1 tabla ; Conventional Sanger sequencing of PCR products is the gold standard for species authentication of seafood products. However, this method is inappropriate for the analysis of products that might contain mixtures of species, such as tinned tuna. The purpose of this study was to test whether next-generation sequencing (NGS) can be a solution for the authentication of mixed products. Nine tuna samples containing mixtures of up to four species were prepared and subjected to an NGS approach targeting two short cytochrome b gene (cytb) fragments on the Illumina MiSeq platform. Sequence recovery was precise and admixtures of as low as 1% could be identified, depending on the species composition of the mixtures. Duplicate samples as well as two individual NGS runs produced very similar results. A first test of three commercial tinned tuna samples indicated the presence of different species in the same tin, although this is forbidden by EU law ; This work was funded by the European Union INTERREG Atlantic Area Program ("LabelFish", project 2011-1/163) ; Peer reviewed

6 páginas, 2 figuras, 1 tabla ; Mislabelling of food products has recently received a great deal of public scrutiny, but it remains unclear exactly what methods are being utilised in laboratories testing the authenticity of foods. In order to gain insight into the specific area of the analysis of seafood, a questionnaire focussing on the taxonomic groups typically analysed and the techniques utilised was sent to over one hundred accredited laboratories across the UK, Ireland, Spain, Portugal, France and Germany. Forty-five responded positively, demonstrating significant differences in both the species analysed and methods utilised among the countries included in the survey. Indeed, a diversity of methods was employed across laboratories and efforts to harmonise and/or standardise testing were evident only at national scale. This contrasts with the EU wide scale of regulation on seafood labelling, and may lead to inconsistencies in the results produced in countries ; This work was funded by the European Union INTERREG Atlantic Area Program ('LabelFish', project 2011-1/163). Additional support also originated from The Department for Environment, Food and Rural Affairs (DEFRA), in the United Kingdom. ; Peer reviewed