Suchergebnisse

For the US House of Representatives, Cox and McCubbins discover tiny majority-party roll rates and offer them as evidence of majority-party agenda control. However, the observed roll rates are approximately what would result from chance alone or from chance constrained in several natural ways. Besides that, we show that rolls themselves are not evidence of any lapse in partisan agenda control and may even occur as the intended consequence of agenda setting by the majority party. Innovations include a solution to the combinatorial problem of counting all possible rolls, the associated computations, hypothetical examples of strategically advantageous self-induced rolls, and a review of likely real examples of the same.

A considerable theoretical literature argues that if everyone votes sincerely, then an agenda setter has near dictatorial influence on final outcomes, whereas if everyone votes strategically, then an agenda setter's power is considerably reduced. This literature assumes that all feasible agendas are of a special type called amendment agendas. But actual legislative and committee agendas—notably those found in Congress—often are not of this type. Once we expand the domain of feasible agendas to include all types allowed by common parliamentary practice, the influence of agendas on legislative outcomes expands, even with strategic voting. Besides showing with counterexamples that previous results do not extend to a more realistic domain of agendas, we prove some theorems that specify the limits (such as they are) of an agenda setter's power.

Abstract
Background
The anti-diabetic pharmaceutical metformin is frequently analysed in the aquatic environment. Its impact on the fish microbiome is studied to get a deeper knowledge about the consequence of the metformin presence in river systems. Gut microbiome analyses were performed on larval brown trout with metformin including environmental concentrations. Therefore, the fish were exposed to metformin in concentrations of 0, 1, 10, 100, and 1000 µg/L. Especially, the lower metformin concentrations were measured in river waters containing percentages of conditioned wastewater from municipal wastewater treatment plants.

Results
Two complementary molecular biological methods for population analysis targeting the 16S rRNA gene regions V1–V3, i.e.: (1) 16S amplicon sequencing and (2) polymerase chain reaction (PCR) combined with denaturing gradient gel electrophoresis (DGGE). Both analyses demonstrated significant microbiome alterations even at low metformin concentrations being analysed in German rivers. The amplicon sequencing revealed the most distinct shifts in the Firmicutes phylum, or more specifically, within the Bacillales order, which were most affected by metformin exposure. Within the Bacillales order, the Planococcaceae family, which is described to provide essential amino acids for the fish, completely disappeared after metformin treatment. Conversely, the percentage of other bacteria, such as Staphylococcaceae, increased after exposure to metformin. Similarity profiles of the microbiomes could be generated using the Sørensen index calculation after PCR-DGGE analyses and confirmed shifts in the composition of the brown trout intestinal microbiome after metformin exposures. In vitro gene expression analyses of virulence factors from fish pathogens, previously identified in the fish microbiomes DNA extracts, were conducted in the presence or absence of environmentally relevant concentrations. Here, marker genes of Enterococcus faecium, Enterococcus faecalis, and Aeromonas hydrophila were detected and quantified via PCR approaches, firstly. An increased expression of the species-specific virulence genes was observed after normalisation with control data and ribosomal housekeeping genes.

Conclusion
Environmentally relevant concentrations of metformin can alter the composition in gut microbiome of brown trout in different ways. Both, the metformin-induced expression of virulence genes in fish pathogens in vitro and the impact of metformin on the microbiome composition in vivo in larval brown trout open the discussion about a possible long-term effect on the vitality, growth, and development in more mature brown trouts.

Abstract
The frequencies of 6 different facultative pathogenic bacteria of the ESKAPE group (priority list WHO) and a total of 14 antibiotic resistance genes (ARGs) with different priorities for human medicine were quantified in wastewaters of poultry and pig slaughterhouses using molecular biological approaches. Raw sewage from poultry and pig slaughterhouses was found to be contaminated not only with facultative pathogenic bacteria but also with various categories of clinically relevant ARGs, including ARGs against the reserve antibiotics group. The concentration of the different gene targets decreased after on-site conventional biological or advanced oxidative wastewater treatments, but was not eliminated. Hence, the antimicrobial BlueLight (aBL) in combination with a porphyrin photo-sensitizer was studied with ESKAPE bacteria and real slaughterhouse wastewaters. The applied broad LED-based blue light (420–480 nm) resulted in groups of sensitive, intermediate, and non-sensitive ESKAPE bacteria. The killing effect of aBL was increased in the non-sensitive bacteria Klebsiella pneumoniae and Enterococcus faecium due to the addition of porphyrins in concentrations of 10−6 M. Diluted slaughterhouse raw wastewater was treated with broad spectrum aBL and in combination with porphyrin. Here, the presence of the photo-sensitizer enhanced the aBL biocidal impact.

Two studies designed to increase the low production rates of severely retarded workers in a sheltered workshop setting are described. In one, differential reinforcement of higher rate was used to increase the rate of assembly of a cable harness from 13% of the standard rate of normal workers to almost 50% of the norm standard. In the second, a system of self-delivery of reward was used to shorten the time required to assemble a test adapter. The subject was trained successfully to perform a quality control check. The implications of the two studies for sheltered workshop settings are explored.