Suchergebnisse

Background/Aims: Liver biopsy for hepatocellular carcinoma (HCC) detection is largely restricted to small hepatocellular lesions, which are often morphologically challenging, requiring careful distinction between dysplastic nodules (high-grade) and well-differentiated HCC. Methods: We investigated the diagnostic accuracy of a panel of markers (HSP70 G PC3 and GS), previously tested in resection specimens, in a series of liver biopsies of large regenerative nodules (n = 13), low-grade dysplastic nodules (n = 21), high-grade dysplastic nodules (n = 50), very well-differentiated (VWD) (n = 17), well-differentiated (WD-G1) (n = 40) and G2-3 (n = 35) HCC. Results: Almost all cases of large regenerative and low-grade dysplastic nodules did not stain while high-grade dysplastic nodules showed 1 marker (22%) but never 2 or 3. For HCC detection the overall accuracy of marker combination was 60.8% (3 markers) and 78.4% (2 markers) with 100% specificity. When restricted to VWD + WD-G1 HCC the accuracy was 57% (3 markers) and 72.9% (2 markers) with 100% specificity. Conclusions: This panel proved useful to detect well-differentiated HCC in biopsy. Two immunoreactive markers (out of 3) are recommended as the most valuable diagnostic combination for HCC detection. The diagnostic accuracy of the panel could be improved using additional markers, as suggested by studies of expression profiling in other human models. (c) 2009 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved. ; This work was supported by a grant (0620210) from the National R&D Program for Cancer Control, Ministry of Health & Welfare, and by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (R13-2002-054-03004-0), Republic of Korea (Y.N. Park, J.J. Jang and E. Yu) and by the Italian MURST (FIRST 2006). ; Seimiya M, 2008, HEPATOLOGY, V48, P519, DOI 10.1002/hep.22364 ; Abdul-Al HM, 2008, HUM PATHOL, V39, P209, DOI 10.1016/j.humpath.2007.06.004 ; Forner A, 2008, HEPATOLOGY, V47, P97, DOI 10.1002/hep.21966 ; Forner A, 2008, HEPATOLOGY, V47, P5, DOI 10.1002/hep.22152 ; Bioulac-Sage P, 2007, HEPATOLOGY, V46, P740, DOI 10.1002/hep.21743 ; Roncalli M, 2007, HEPATOL RES, V37, pS125, DOI 10.1111/j.1872-034X.2007.00175.x ; Audard V, 2007, J PATHOL, V212, P345, DOI 10.1002/path.2169 ; Di Tommaso L, 2007, HEPATOLOGY, V45, P725, DOI 10.1002/hep.21531 ; Park YN, 2007, CANCER, V109, P915, DOI 10.1002/cncr.22460 ; HYTIROGLOU P, 2007, GASTROENTEROL CLIN N, V36, P867 ; Liu L, 2006, CANCER RES, V66, P11851, DOI 10.1158/0008-5472.CAN-06-1377 ; Llovet JM, 2006, GASTROENTEROLOGY, V131, P1758, DOI 10.1053/j.gastro.2006.09.014 ; Yamauchi N, 2005, MODERN PATHOL, V18, P1591, DOI 10.1038/modpathol.3800436 ; Bruix J, 2005, HEPATOLOGY, V42, P1208, DOI 10.1002/hep20933 ; Lorinc E, 2005, HISTOPATHOLOGY, V46, P642, DOI 10.1111/j.1365-2559.2005.02139.x ; Kojiro M, 2005, SEMIN LIVER DIS, V25, P133 ; Dray M, 2005, PATHOLOGY, V37, P112, DOI 10.1080/00313020500058607 ; RONCALLI M, 2004, LIVER TRANSPLANT, V10, pS9 ; Paradis V, 2003, AM J PATHOL, V163, P733 ; Capurro M, 2003, GASTROENTEROLOGY, V125, P89, DOI 10.1016/S0016-5085(03)00689-9 ; Chuma M, 2003, HEPATOLOGY, V37, P198, DOI 10.1053/jhep.2003.50022 ; Sparrow LE, 1998, AM J DERMATOPATH, V20, P255 ; CHRISTA L, 1994, GASTROENTEROLOGY, V106, P1312 ; 34