Suchergebnisse

The legislation on environmental contaminants in food is constantly evolving. Some of these contaminants have already been the subject of official control of the food chain, others, such as polychlorinated biphenyls, are newly added, while others, such as organometallic compounds, are not yet covered. Present work is based on the development of innovative techniques aimed at the implementation of routine analytical methods for the control of substances recently and probable future inclusion in the Official monitoring plans, such as non dioxin-like polychlorinated biphenyls (NDL-PCB) and compounds of arsenic in highly contaminated products such as fishery products. Regarding the NDL-PCB, this work aims to focus on validation study of an analytical screening method, aimed to detection of polychlorinated biphenyls (PCBs) in fish intended for human consumption, as required by Commission Decision 657/2002/EC. Regarding arsenic, the Committee of Experts of the FAO/WHO defined a tolerable intake only for inorganic arsenic, although it is known the toxicity of some organoarsenic compounds; here we present the validation study of a new method for a rapid and reliable speciation of these compounds in mussels. This work has allowed us to achieve several goals. Firstly, it has been provided an example, so far not present in the scientific literature, of application of the European requirements for accreditation of the multiresidual screening method for NDL-PCBs determination, whose validity has been proved obtaining the recognition by the Italian accreditation body "Accredia". Moreover, guidance to the regulation has been also provided by the inclusion of substances characterized by overt toxicity and evident presence in foods of animal origin, which are not covered by national and European legislation, such as the various forms of arsenic; moreover, the analytical solutions to laboratories for Official control were provided.

AbstractThis study assesses the PAH and heavy metal levels in muscle of warty crabs (Eriphia verrucosa), from the northern coast of the Campania region improving the data on toxic contaminants in this crustacean. The results showed a minimal PAH contamination; the mean concentrations were as follows: 0.2, 1.6 and 1.7 μg kg-1 wet weight (ww) for BaP, PAH4 and PAH6, respectively. Regarding the levels of the two PAHs not included in the European regulations, the BkF mean concentration was 0.1 μg kg-1 ww, while DahA was detected only in 10.7% of samples. Pb and Cr were also detected at low levels with mean values of 0.068 and 0.468 mg kg-1 ww, respectively; instead, high As levels, with a mean value of 5.021 mg kg-1 ww, were found. Considering the EWIs and the ILCRs calculated in this study, the PAH, Pb and Cr contamination levels found in the edible part of the crabs resulted safe for human consumption. Contrariwise, the ILCR calculated for the As exceeded the acceptable level of cancer risk, although the calculation did not refer to the inorganic form which is the only one recognized as carcinogenic. Hence, this study shows that warty crabs can accumulate environmental contaminants in their muscle tissue representing an important route of exposure to these toxics for the local population that regularly consumes them. This finding highlights the importance of monitoring the presence of these pollutants in crabs and in general in all fish and seafood in order to ensure food safety for consumers.

SIMPLE SUMMARY: Buffalo mozzarella cheese, sold as a Protected Designation of Origin (PDO) product, is made exclusively from Mediterranean buffalo (Bubalus bubalis) milk. To maximize their profits and overcome seasonal shortages of buffalo milk, some producers have started to produce "PDO" buffalo mozzarella cheese by adulterating buffalo milk with milk from different species. such as bovine, thus defrauding consumers. This practice has led the Italian government to reinforce controls on food mislabeling and fraud through traceability mechanisms. The aim of this work is the development of a molecular assay—droplet digital Polymerase Chain Reaction technique (ddPCR)—able to detect the DNA of cow and/or buffalo milk in PDO buffalo mozzarella cheese, thus revealing fraud. The results have highlighted that, thanks to its high precision and sensitivity, the ddPCR could represent an efficacious means of supporting the official controls aimed at combating the adulteration of buffalo mozzarella cheese with bovine milk. ABSTRACT: Buffalo mozzarella cheese is one of the most appreciated traditional Italian products and it is certified as a Protected Designation of Origin (PDO) product under the European Commission Regulation No. 1151/2012. It is obtained exclusively from buffalo milk. If made from cow milk, or a mixture of buffalo and cow milk, buffalo mozzarella cheese does not qualify as a PDO product. In order to maximize their profits, some producers market buffalo mozzarella that also contains cow milk as a PDO product, thus defrauding consumers. New methods for revealing this fraud are therefore needed. One such method is the droplet digital Polymerase Chain Reaction (ddPCR). Thanks to its high precision and sensitivity, the ddPCR could prove an efficacious means for detecting the presence of cow milk in buffalo mozzarella cheese that is marketed as a PDO product. ddPCR has proved able to detect the DNA of cow and/or buffalo milk in 33 buffalo mozzarella cheeses labelled as PDO products, and experimental evidence ...

This study reports the results of aflatoxins B/G monitoring in food of vegetal origin, imported in Southern Italy from extra-European Union countries. From 2017 to 2020, we analyzed 1675 samples using an accredited HPLC method with fluorescence detection. We found out 295 samples (17.6%) were contaminated by aflatoxin B1, 204 by aflatoxins B/G (12.2%), while 75 (4.5%) resulted non-compliant to maximum limits set by the European Union law. Most of the batches tested were unprocessed food; the distribution of contamination levels, incidence of non-compliant samples, inference for different kinds of food are reported. The study focuses on the food more susceptible to contamination by aflatoxins; nuts are the food more controlled, showing the higher number of non-compliant samples. Our study confirms that pistachio nuts, hazelnuts and almonds are the major sources of exposure for consumers. Still, other products, such as chili pepper and Brazil nuts, need to get more information about their contamination levels. The study's findings are discussed in the perspective of the last opinion by EFSA about chronic exposure to aflatoxins. A case study to evaluate not compliance of a composed food to the European Union law is reported.