Suchergebnisse

Jun Hou,1 Donghui Fan,2 Lingming Zhao,2 Baoqin Yu,3 Jiacan Su,3 Jie Wei,2 Jung-Woog Shin4 1The First Affiliated Hospital of Anhui Medical University, Department of Oral and Maxillofacial Surgery, Hefei, 2Key Laboratory for Ultrafine Materials of Ministry of Education and The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 3Department of Orthopaedics Trauma, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China; 4Department of Biomedical Engineering, Inje University, Gimhae, Republic of Korea Abstract: Biocomposite scaffolds were fabricated by incorporation of nanobredigite (n-BD) into the polymer of poly(ε-caprolactone)–poly(ethyleneglycol)–poly(ε-caprolactone) (PCL–PEG–PCL). The results revealed that the addition of n-BD into PCL–PEG–PCL significantly improved water absorption, compressive strength, and degradability of the scaffolds of n-BD/PCL–PEG–PCL composite (n-BPC) compared with PCL–PEG–PCL scaffolds alone. In addition, the proliferation and alkaline phosphatase activity of MG63 cells cultured on n-BPC scaffolds were obviously higher than that cultured on PCL–PEG–PCL scaffolds. Moreover, the results of the histological evaluation from the animal model revealed that the n-BPC scaffolds significantly improved new bone formation compared with the PCL–PEG–PCL scaffolds, indicating good osteogenesis. The n-BPC scaffolds with good biocompatibility could stimulate cell proliferation, differentiation, and bone tissue regeneration and would be an excellent candidate for bone defect repair. Keywords: nanobredigite, PCL–PEG–PCL, biocomposite scaffolds, degradability, osteo­genesis

Yun Gyeong Kang,1 Jie Wei,2 Ji Won Shin,1 Yan Ru Wu,3 Jiacan Su,4 Young Shik Park,5 Jung-Woog Shin1,3,6 1School of Biomedical Engineering, Inje University, Gimhae, Republic of Korea; 2Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai, China; 3Department of Health Science and Technology, Inje University, Gimhae, Republic of Korea; 4Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai, China; 5School of Biological Science, Inje University, Gimhae, Republic of Korea; 6Cardiovascular and Metabolic Disease Center/Institute of Aged Life Redesign/UHARC, Inje University, Gimhae, Republic of Korea Background: Successful bone tissue engineering using scaffolds is primarily dependent on the properties of the scaffold, including biocompatibility, highly interconnected porosity, and mechanical integrity. Methods: In this study, we propose new composite scaffolds consisting of mesoporous magnesium silicate (m_MS), polycaprolactone (PCL), and wheat protein (WP) manufactured by a rapid prototyping technique to provide a micro/macro porous structure. Experimental groups were set based on the component ratio: (1) WP0% (m_MS:PCL:WP =30:70:0 weight per weight; w/w); (2) WP15% (m_MS:PCL:WP =30:55:15 w/w); (3) WP30% (m_MS:PCL:WP =30:40:30 w/w). Results: Evaluation of the properties of fabricated scaffolds indicated that increasing the amount of WP improved the surface hydrophilicity and biodegradability of m_MS/PCL/WP composites, while reducing the mechanical strength. Moreover, experiments were performed to confirm the biocompatibility and osteogenic differentiation of human mesenchymal stem cells (MSCs) according to the component ratio of the scaffold. The results confirmed that the content of WP affects proliferation and osteogenic differentiation of MSCs. Based on the last day of the experiment, ie, the 14th day, the proliferation based on the amount of DNA was the best in the WP30% group, but all of the markers measured by PCR were the most expressed in the WP15% group. Conclusion: These results suggest that the m_MS/PCL/WP composite is a promising candidate for use as a scaffold in cell-based bone regeneration. Keywords: mesoporous magnesium silicate, wheat protein, scaffold, bone tissue engineering, osteogenic differentiation

Liehu Cao,1,* Weizong Weng,1,* Xiao Chen,1,* Jun Zhang,1 Qirong Zhou,1 Jin Cui,1 Yuechao Zhao,1 Jung-Woog Shin,2 Jiacan Su1 1Department of Orthopaedics Trauma, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China; 2Department of Biomedical Engineering, Inje University, Gimhae, Republic of Korea *These authors contributed equally to this work Abstract: Nanoporous lithium doping magnesium silicate (nl-MS) was introduced into calcium sulfate hemihydrate to prepare calcium sulfate composite (nl-MSC) bone cements. The introduction of nl-MS improved the in vitro degradability of nl-MSC cements, which could neutralize acidic degradable products of calcium sulfate and prevented the pH from dropping. The cements were implanted into the bone defects of femur bone of rabbits, and the results of histological and immunohistochemical analysis revealed that massive new bone tissue formed in the defects while the cements were degradable, indicating that the osteogenesis and degradability of the nl-MSC cements were much better than the control calcium sulfate dihydrate (CSD) cements. Furthermore, the positive expression of vascular endothelial growth factor and collagen type I for nl-MSC cements was higher than CSD, indicating that addition of nl-MS into the cements enhanced vascularization and osteogenic differentiation. The results suggested that the nl-MSC cements with good biocompatibility and degradability could promote vascularization and osteogenesis, and had great potential to treat bone defects. Keywords: bone cements, nanoporous lithium doping magnesium silicate, degradability, vascularization, osteogenesis

Zhaoying Wu,1 Quan Li,2 Yongkang Pan,1 Yuan Yao,1 Songchao Tang,1 Jiacan Su,2 Jung-Woog Shin,3 Jie Wei,1 Jun Zhao4,5 1Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, 2Department of Orthopaedics Trauma, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China; 3Department of Biomedical Engineering, Inje University, Gimhae, Republic of Korea; 4Department of Orthodontics, 5Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai, People's Republic of China Abstract: Bioactive composite macroporous scaffold containing nanoporosity was prepared by incorporation of nanoporous magnesium silicate (NMS) into poly(butylene succinate) (PBSu) using solvent casting–particulate leaching method. The results showed that the water absorption and in vitro degradability of NMS/PBSu composite (NMPC) scaffold significantly improved compared with magnesium silicate (MS)/PBSu composite (MPC) scaffold. In addition, the NMPC scaffold showed improved apatite mineralization ability, indicating better bioactivity, as the NMPC containing nanoporosity could induce more apatite and homogeneous apatite layer on the surfaces than MPC scaffold. The attachment and proliferation of MC3T3-E1 cells on NMPC scaffold increased significantly compared with MPC scaffold, and the alkaline phosphatase (ALP) activity of the cells on NMPC scaffold was expressed at considerably higher levels compared with MPC scaffold. Moreover, NMPC scaffold with nanoporosity not only had large drug loading (vancomycin) but also exhibited drug sustained release. The results suggested that the incorporation of NMS into PBSu could produce bioactive composite scaffold with nanoporosity, which could enhance water absorption, degradability, apatite mineralization and drug sustained release and promote cell responses. Keywords: nanocomposite, degradability, apatite mineralization, cell behaviors, drug release