Open Access#12016
Specific serology for emerging human coronaviruses by protein microarray
We present a serological assay for the specific detection of IgM and IgG antibodies against the emerging human coronavirus hCoV-EMC and the SARS-CoV based on protein microarray technology. The assay uses the S1 receptor-binding subunit of the spike protein of hCoV-EMC and SARS-CoV as antigens. The assay has been validated extensively using putative cross-reacting sera of patient cohorts exposed to the four common hCoVs and sera from convalescent patients infected with hCoV-EMC or SARS-CoV. ; RdS was funded by the European Public Health Microbiology Training Program (EUPHEM), ECDC, Stockholm. LvdH, BH and AO were funded by the European Union FP7 project EMPERIE (contract number 223498).