Suchergebnisse

Pan Shang,1,* Gan Chen,1,* Guannan Zu,2 Xiang Song,1 Peng Jiao,2 Guoxing You,1 Jingxiang Zhao,1 Hongyi Li,2 Hong Zhou11Academy of Military Medical Sciences, Institute of Health Service and Transfusion Medicine, Beijing 100850, People's Republic of China; 2Photoelectrochemical Research Group, School of Materials Science and Engineering, Beijing University of Technology, Beijing 100124, People's Republic of China *These authors contributed equally to this workBackground: Cardiovascular disease (CVD) is the leading cause of mortality all over the world. Vascular stents are used to ameliorate vascular stenosis and recover vascular function. The application of nanotubular coatings has been confirmed to promote endothelial cell (EC) proliferation and function. However, the regulatory mechanisms involved in cellular responses to the nanotubular topography have not been defined. In the present study, a microarray analysis was performed to explore the expression patterns of long noncoding RNAs (lncRNAs) in human coronary artery endothelial cells (HCAECs) that were differentially expressed in response to nitinol-based nanotubular coatings.Materials and methods: First, anodization was performed to synthesize nitinol-based nanotubular coatings. Then, HCAECs were cultured on the samples for 24 h to evaluate cell cytoskeleton organization. Next, total RNA was extracted and synthesized into cRNA, which was hybridized onto the microarray. GO analysis and KEGG pathway analysis were performed to investigate the roles of differentially expressed messenger RNAs (mRNAs). Quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) was performed to validate the expression of randomly selected lncRNAs. Coexpression networks were created to identify the interactions among lncRNAs and the protein-coding genes involved in nanotubular topography-induced biological and molecular pathways. Independent Student's t-test was applied for comparisons between two groups with statistical significance set at p<0.05.Results: 1085 lncRNAs and 227 mRNAs were significantly differentially expressed in the nitinol-based nanotubular coating group. Bioinformatics analysis revealed that extracellular matrix receptor interactions and cell adhesion molecules play critical roles in the sensing of nitinol-based nanotubular coatings by HCAECs. The TATA-binding protein (TBP) and TBP-associated transfactor 1 (TAF1) are important molecules in EC responses to substrate topography.Conclusion: This study suggests that nanotubular substrate topography regulates ECs by differentially expressed lncRNAs involved extracellular matrix receptor interactions and cell adhesion molecules.Keywords: nitinol titanium dioxide nanotubes, RNA sequencing, long noncoding RNA, nanotopography, molecular networks

AbstractN,S‐codoped mesoporous carbon (S/NMC) as an effective catalyst toward the catalytic persulfate oxidation of sulfamethazine (SMZ) was fabricated conveniently using vinasse as the C/N source, Na2S as the S precursor and nano‐SiO2 as the template, respectively. The effect of S precursor dosage on the textural properties and surface chemistry of S/NMCs were characterized by N2 adsorption/desorption, Raman spectra, X‐ray diffractometer (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT‐IR) and X‐ray photoelectron spectroscopy (XPS). It was found that more S precursor dosage presented an enhanced SMZ degradation performance due to the significant role of more S precursor dosage: (i) larger specific surface area and (ii) more active surface groups including pyridine N, graphite N, quinone‐like O and thiophene‐like S. In addition, quenching experiments showed that free radical and nonradical oxidation processes are the main processes of SMZ degradation. The findings provided a new idea for the resource utilization of vinasse and the development of catalytic persulfate oxidation of organic pollution.