Suchergebnisse

[Background] Sarcoptic mange has been identified as the most significant infectious disease affecting the Iberian ibex (Capra pyrenaica). Despite several studies on the effects of mange on ibex, the pathological and clinical picture derived from sarcoptic mange infestation is still poorly understood. To further knowledge of sarcoptic mange pathology, samples from ibex were evaluated from histological, microbiological and serological perspectives. ; [Methods] Samples of skin, non-dermal tissues and blood were collected from 54 ibex (25 experimentally infected, 15 naturally infected and 14 healthy). Skin biopsies were examined at different stages of the disease for quantitative cellular, structural and vascular changes. Sixteen different non-dermal tissues of each ibex were taken for histological study. Acetylcholinesterase and serum amyloid A protein levels were evaluated from blood samples from ibex with different lesional grade. Samples of mangy skin, suppurative lesions and internal organs were characterized microbiologically by culture. Bacterial colonies were identified by a desorption/ionization time-of-flight mass spectrometry system (MALDI TOF/TOF). ; [Results] The histological study of the skin lesions revealed serious acanthosis, hyperkeratosis, rete ridges, spongiotic oedema, serocellular and eosinophilic crusts, exocytosis foci, apoptotic cells and sebaceous gland hyperplasia. The cellular response in the dermis was consistent with type I and type IV hypersensitivity responses. The most prominent histological findings in non-dermal tissues were lymphoid hyperplasia, leukocytosis, congestion and the presence of amyloid deposits. The increase in serum concentrations of acetylcholinesterase and amyloid A protein correlated positively with the establishment of the inflammatory response in mangy skin and the presence of systemic amyloidosis. A wide variety of bacterial agents were isolated and the simultaneous presence of these in mangy skin, lymph nodes and internal organs such as lungs, liver, spleen and kidney was compatible with a septicaemic pattern of infection. ; [Conclusions] The alteration of biomarkers of inflammation and its implication in the pathogenesis of the disease and development of lesions in non-dermal tissues and septicaemic processes are serious conditioners for the survival of the mangy ibex. This severe clinical picture could be an important factor when considering the decision to eliminate animals that exceed a certain disease threshold from a population. ; This study was funded by MINECO from the Spanish Government (grant numbers CGL2012–40043-C0–01, CGL2012–40043-CO2–02 and CGL2016–80543-P). The authors' research activities are partially funded by the PAIDI Research Group RNM-118 from Junta de Andalucia. José Espinosa was supported by a PhD Grant (grant number ECC/1402/2013: BES-2013-063931). This study is part of the project "Bases biológicas para la gestión de la sarna sarcóptica en la cabra montés (Capra pyrenaica) de Sierra Nevada". ; Peer reviewed

Plant primary production is a key driver of several ecosystem functions in seasonal marshes, such as water purification and secondary production by wildlife and domestic animals. Knowledge of the spatio-temporal dynamics of biomass production is therefore essential for the management of resources—particularly in seasonal wetlands with variable flooding regimes. We propose a method to estimate standing aboveground plant biomass using NDVI Land Surface Phenology (LSP) derived from MODIS, which we calibrate and validate in the Doñana National Park's marsh vegetation. Out of the different estimators tested, the Land Surface Phenology maximum NDVI (LSP-Maximum-NDVI) correlated best with ground-truth data of biomass production at five locations from 2001–2015 used to calibrate the models (R 2 = 0.65). Estimators based on a single MODIS NDVI image performed worse (R 2 ≤ 0.41). The LSP-Maximum-NDVI estimator was robust to environmental variation in precipitation and hydroperiod, and to spatial variation in the productivity and composition of the plant community. The determination of plant biomass using remote-sensing techniques, adequately supported by ground-truth data, may represent a key tool for the long-term monitoring and management of seasonal marsh ecosystems. ; We acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI). ; David Aragonés, Isabel Afán, Ricardo Díaz-Delgado and Diego García Díaz (EBD-LAST) provided support for remote-sensing and LSP analyses. Alfredo Chico, José Luis del Valle and Rocío Fernández Zamudio (ESPN, ICTS-RBD) provided logistic support and taxonomic expertise during the field work (validation dataset). Ernesto García and Cristina Pérez assisted with biomass harvesting and processing (calibration dataset). Gerrit Heil provided support in the project design. This study received funding from Ministerio de Medio Ambiente-Parque Nacional de Doñana, Consejeria de Medio Ambiente, Junta de Andalucia (1999–2000): RNM118 Junta de Andalucia (2003); the European Union's Horizon 2020 Research and Innovation Program under grant agreement No. 641762 to ECOPOTENTIAL project; and the Spanish Ministry of Economy, Plan Estatal de I+D+i 2013–2016, under grant agreement CGL2016-81086-R to GRAZE project.

Abstract Background Wildlife radio tracking has gained popularity during the recent past. Ecologists and conservationists use radio-collars for different purposes: animal movement monitoring, home range, productivity, population estimation, behaviour, habitat use, survival, and predator-prey interaction, among others. The aim of our present study is to highlight the application of radio-collars for wildlife diseases monitoring. The spread of wildlife diseases and the efficacy of management actions for controlling them propose serious challenges for ecologists and conservationists, since it is difficult to re-capture (or simply observe) the same animal in pre-determined temporal interval, but such difficulty is overcome by the use of gps-gsm radio collars. Methods In the present study we report, for the first time to our knowledge, the use of radio-collars in the monitoring of Iberian ibex affected by Sarcoptes scabiei in Sierra Nevada mountain range, Spain. Twenty-five moderate or slightly mangy animals were radio-collared between 2006 and 2013. Results The radio-collars allowed us to confirm the presence of resistance to S. scabiei within Iberian ibex population. Twenty (80%) of the collared animals recovered totally from mange, while the disease progressed in the other five Iberian ibex (20% of the collared animals) and the animals died. The average estimated recovery time of the resistant animals was 245 ± 277 days, and the estimated average survival time of the non-resistant Iberian ibex was 121 ± 71 days. Non-resistant animals survived at least 100 days, while all of them died with less than 200 days. Sixty per cent of the resistant animals were recovered with less than 200 days. Conclusions We report, for the first time, the successful use of radio collars for wildlife diseases monitoring using Iberian ibex/S. scabiei as a model. By using radio collars we documented that most of the Sarcoptes-infected Iberian ibex are resistant to this disease, and we estimated the average time for Iberian ibex recovering from mange infection and the average survival time of the non-resistant ones. We expect wider use of radio-collars for wild animals diseases monitoring, affected/not-affected animals interaction, and treatment efficacy, among others. ; The research was supported by the projects 173/2009/M/00 and 861/11/M/00 (CMA, Andalusian Government), CGL2004-03171 and CGL2012-40043-C02-01 (MEC, Spanish Goverment), RTA 2009-00114-00-00 (INIA), RNM-6400, and Proyecto de Excelencia (Junta de Andalucia, Spain), together with Juan de la Cierva grant. The authors also thank the Unit of Information Resources for Research (URICI-CSIC) for the co-financing of this publication in Open Access. ; The authors also thank the Unit of Information Resources for Research (URICI-CSIC) for the co-financing of this publication in Open Access. ; Peer Reviewed

This dataset provides long-term information on the presence of the Iberian ibex (Capra pyrenaica hispanica Schimper, 1848) in Sierra Nevada (SE Iberian Peninsula). Data on the abundance and demographic structure of the Iberian ibex population were compiled over the last three decades. Transects were laid out to record different variables such as the number of individuals sighted, the perpendicular distance of each group of Iberian ibex to the transect line and sex as well as age of individuals in the case of males. These data enabled the calculation of population parameters such as density, sex ratio, birth rate, and age structure. These parameters are key for Iberian ibex conservation and management, given that Sierra Nevada harbours the largest population of this species in the Iberian Peninsula. The data set we present is structured using the Darwin Core biological standard, which contains 3,091 events (582 transect walk events and 2,509 group sighting events), 5,396 occurrences, and 2,502 measurements. The occurrences include the sightings of 11,436 individuals (grouped by sex and age) from 1993 to 2018 in a total of 88 transects distributed along Sierra Nevada, of which 33 have been continuously sampled since 2008. ; Projects "LIFE-ADAPTAMED: Protection of key ecosystem services by adaptive management of Climate Change endangered Mediterranean socioecosystems" LIFE14 CCA/ES/000612 ; H2020 project "ECOPOTENTIAL: Improving future ecosystem benefits through earth observations" - European Union's Horizon 2020 research and innovation programme 641762 ; Elter H2020 project "European Long-Term Ecosystem and Socio-Ecological Research Infrastructure" - European Union's Horizon 2020 programme 654359 ; 10010003 ; 10050010 ; 10040016 ; 676/2006/A/00 ; 1571/2007/M/00 ; 173/2009/M/00 ; 861/11/M/00 ; 03/15/M/00 ; 2016 _00014_M ; 2017-00189M

This study was funded by MINECO from the Spanish Government (grant numbers CGL2012-40043-C0-01, CGL2012-40043-CO2-02 and CGL2016-80543-P). The authors' research activities are partially funded by the PAIDI Research Group RNM-118 from Junta de Andalucia. José Espinosa was supported by a PhD Grant (grant number ECC/1402/2013: BES-2013-063931). This study is part of the project "Bases biológicas para la gestión de la sarna sarcóptica en la cabra montés (Capra pyrenaica) de Sierra Nevada". ; Sarcoptic mange has been identified as the most significant infectious disease affecting the Iberian ibex (Capra pyrenaica). Despite several studies on the effects of mange on ibex, the pathological and clinical picture derived from sarcoptic mange infestation is still poorly understood. To further knowledge of sarcoptic mange pathology, samples from ibex were evaluated from histological, microbiological and serological perspectives.

In order to define the spatial interface between wild boar and domestic pigs in Europe, the ENETWILD consortium (www.enetwild.com) described in a preliminary report the different sources of data for domestic pigs at European scale, and developed a preliminary risk map of possible spatial interaction between both groups. This modelexplored and assessed the use of pig distribution data from Gridded Livestock of the Worlddatabase (GLW), FAO. However, in some specific countries used as cases, the GLW predictions did not reliably represent the pig abundance distribution within countries. The currently available census data of livestock at the European Union level (Eurostat) is limited to the spatial resolution at NUTS2. While Eurostat ensures that data can be potentially comparable,there is still needed to resolve definition issues regarding better spatial resolution (level of aggregation of information) and the pig production systems. In this context, the objectives of this report are (i) assessing the spatial interface between pigs and wild boar over Europe using the best quality data available (Eurostat data and ENETWILD spatial models). We(ii) secondly assessed the interface at higher spatial resolution, distinguishing pig production types in countries where data was available. Based on comparisons at different scales and quality of data, we propose future steps in both data collection and modelling approach.Precisespatial resolution of pig data is not available at European level yet, and the discrimination of extensive vs. intensive farms, backyards vs. commercial; outdoor vs. indoor, is essential to quantify and perform risk analyses separatelyfor each production system and/or considering this relevant source of variation in risk at the interface. The development of a framework to collect harmonised and standardised data at European scale athigher resolution is needed. ; Peer reviewed

Abstract Background Defining hidden genetic diversity within species is of great significance when attempting to maintain the evolutionary potential of natural populations and conduct appropriate management. Our hypothesis is that isolated (and eventually small) wild animal populations hide unexpected genetic diversity due to their maintenance of ancient polymorphisms or introgressions. Results We tested this hypothesis using the Iberian ibex (Capra pyrenaica) as an example. Previous studies based on large sample sizes taken from its principal populations have revealed that the Iberian ibex has a remarkably small MHC DRB1 diversity (only six remnant alleles) as a result of recent population bottlenecks and a marked demographic decline that has led to the extinction of two recognized subspecies. Extending on the geographic range to include non-studied isolated Iberian ibex populations, we sequenced a new MHC DRB1 in what seemed three small isolated populations in Southern Spain (n = 132). The findings indicate a higher genetic diversity than previously reported in this important gene. The newly discovered allele, MHC DRB1*7, is identical to one reported in the domestic goat C. aegagrus hircus. Whether or not this is the result of ancient polymorphisms maintained by balancing selection or, alternatively, introgressions from domestic goats through hybridization needs to be clarified in future studies. However, hybridization between Iberian ibex and domestic goats has been reported in Spain and the fact that the newly discovered allele is only present in one of the small isolated populations and not in the others suggests introgression. The new discovered allele is not expected to increase fitness in C. pyrenaica since it generates the same protein as the existing MHC DRB1*6. Analysis of a microsatellite locus (OLADRB1) near the new MHC DRB1*7 gene reveals a linkage disequilibrium between these two loci. The allele OLADRB1, 187 bp in length, was unambiguously linked to the MHC DRB1*7 allele. This enabled us to perform a DRB-STR matching method for the recently discovered MHC allele. Conclusions This finding is critical for the conservation of the Iberian ibex since it directly affects the identification of the units of this species that should be managed and conserved separately (Evolutionarily Significant Units). ; We would like to thank Apolo Sánchez, Elías Martínez, Isidro Puga, Pepe López, Francisco Casado and Antonio Rodríguez for their assistance with the fieldwork in Sierra Nevada. We are also grateful to Miguel Ángel Habela, Santiago Lavín, Pelayo Acevedo, Eularico Fernández, Francisco Martínez, Juan Monje, Jaime Medina, Juan Antonio Funes, Antonio Serrano, Pepe Madrazo, Luis Alfonso Sarmiento and José Luis Chao for providing Iberian ibex samples. ; This study was partially funded by the Consejería de Medio Ambiente of the Junta de Andalucía (projects 173/2009/M/00 and 03/15/M/00) and the Ministerio de Economía y Competitividad of the Spanish Government (projects CGL2012–40043- C02–01, CGL2012–40043-C02–02 and CGL2016– 80543-P). The funding bodies did not contribute to the design of the study or collection, analysis and interpretation of data, or to the writing of the manuscript.