Suchergebnisse

In response to various legislative mandates the United States Environmental Protection Agency (USEPA) formed its Endocrine Disruptor Screening Program (EDSP), which in turn, implemented a tiered testing strategy to determine the potential of pesticides, commercial chemicals, and environmental contaminants to disrupt the endocrine system. The first tier of tests is intended to detect the potential for endocrine disruption mediated through estrogen, androgen or thyroid pathways, while the second tier is intended to further characterize the effects on these pathways and to establish a dose-response relationship for adverse effects. One of these Tier 2 tests, the Medaka Extended One Generation Reproduction Test (MEOGRT) was developed by the USEPA for the EDSP and, in collaboration with the Japanese Ministry of the Environment, for the Organisation for Economic Co-operation and Development's (OECD) Guidelines for the Testing of Chemicals. The MEOGRT protocol was iteratively modified based upon knowledge gained after successfully completing nine tests with variations in test protocols. The current manuscript describes both the final MEOGRT protocol that has been published by the USEPA and the OECD, the iterations of the protocol that provided valuable insights into nuances of the protocol, and the datasets and summaries from the various tests that informed the decision making process. The various tests include exposure to 17β-estradiol, 4-t-octylphenol, o,p'- dichlorodiphenyltrichloroethane, 4-chloro-3-methylphenol, tamoxifen, 17β-trenbolone, vinclozolin, and prochloraz.