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World Affairs Online
Response latency in interest assessment: An added tool?
In: Journal of vocational behavior, Band 108, S. 121-131
ISSN: 1095-9084
Average urinary flow rate and its association with handgrip strength
In: The aging male: the official journal of the International Society for the Study of the Aging Male, Band 23, Heft 5, S. 1220-1226
ISSN: 1473-0790
Coping with workplace minority stress: Associations between dyadic coping and anxiety among women in same-sex relationships
In: Journal of lesbian studies, Band 21, Heft 1, S. 70-87
ISSN: 1540-3548
Stress Spillover and Crossover in Same-Sex Couples: Concurrent and Lagged Daily Effects
In: Journal of GLBT family studies, Band 13, Heft 3, S. 236-256
ISSN: 1550-4298
Complexes containing cationic and anionic pH-sensitive liposomes: comparative study of factors influencing plasmid DNA gene delivery to tumors
In: info:eu-repo/semantics/altIdentifier/doi/10.2147/IJN.S42800
Yan Chen,* Ji Sun,* Ying Lu, Chun Tao, Jingbin Huang, He Zhang, Yuan Yu, Hao Zou, Jing Gao, Yanqiang Zhong Department of Pharmaceutical Science, School of Pharmacy, The Second Military Medical University, Shanghai, People's Republic of China *These authors contributed equally to this work Abstract: pH-sensitive liposomes represent an effective gene vector in cancer therapy. However, their use is greatly hampered by their relatively low transfection efficiency. To improve the transfection efficiency of pH-sensitive liposomes, we prepared complexes containing 3β-[N-(N',N'-dimethylaminoethane) carbamoyl] cholesterol (DC-Chol) and dioleoylphosphatidyl ethanolamine (DOPE) liposomes and pH-sensitive liposomes composed of cholesteryl hemisuccinate (CHEMS) and DOPE, and evaluated the influence of various factors on plasmid DNA (pDNA) transfection efficiency. All DC-Chol/DOPE liposome/pDNA and pH-sensitive liposome complexes showed similarly potent pH sensitivity. In the presence of serum-containing medium, two optimized complexes of DC-Chol/DOPE liposomes/pDNA and pH-sensitive PEGylated liposomes showed high transfection efficiency of 22.94% and 20.07%, respectively. Notably, DC-Chol/DOPE (2:3) liposomes/pH-sensitive PEGylated (1%) liposome complexes with a charge ratio of 1:1 (m/m [+/-]) showed enhanced accumulation in tumors in vivo. Our results show the influence of various factors on pDNA transfection efficiency in complexes of DC-Chol/DOPE liposomes and pH-sensitive PEGylated liposomes. Understanding of such mechanisms will lead to better design of complexes of DC-Chol/DOPE liposomes and pH-sensitive liposomes for gene therapy. Keywords: cationic liposomes, pH-sensitive liposomes, pDNA, transfection, PEGylated
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