Introduction. The article is devoted to identifying features of spatial placement of health clinics in megacities and assessing the degree of compliance/ deviation with standards of pedestrian accessibility. Methods. The theoretical and methodological basis of the research is scientific ideas in the field of regional economics, spatial and normative analysis. Scientific novelty of the research consists in as- sessing availability and saturation of territories by health clinics, in the context of features of their spatial placement, taking into account the estab- lished regulatory requirements. Results. The place of the megacities of Ural Federal District among the megacities of Russia has been determined according to the conditions affecting availability of health clinics for the population. When carrying out a cartographic analysis of spatial placement of health clinics in Yekaterinburg and Chelyabinsk, district features of the cities studied were established, which made it possible to identify the existing problem areas characterized by an im- balance in the level of infrastructure facilities acces- sibility in relation to the regulatory requirements. The authors show the uneven coverage of clinic service areas, which reduces pedestrian and transport accessibility for citizens when receiving medical services. The features and differences of territorial localization of clinics in the actively built- up areas of Yekaterinburg and Chelyabinsk are revealed. Conclusions. The results obtained can be applied in the framework of determining priorities for implementing spatial transformations of studied territories and the authorities' decision-making in order to increase medical services availability.
This research was funded by the European Union Framework Program 7, Project BacHBerry [FP7–613793]. The authors also acknowledge support from the Institute Strategic Programmes 'Designing Future Wheat' (BB/P016855/1), 'Understanding and Exploiting Plant and Microbial Secondary Metabolism' (BB/J004596/1) and 'Molecules from Nature' (BB/P012523/1) from the UK Biotechnology and Biological Sciences Research Council to the John Innes Centre and the European funded COST ACTION FA1106 QualityFruit. VT, PV and CM have also received funding from the European Union's Horizon 2020 research and innovation programme through the TomGEM project under grant agreement No. 679796. The funding bodies had no role in the design of the study, collection, analysis and interpretation of data nor in writing the manuscript. ; Background: Flavonoids are produced in all flowering plants in a wide range of tissues including in berry fruits. These compounds are of considerable interest for their biological activities, health benefits and potential pharmacological applications. However, transcriptomic and genomic resources for wild and cultivated berry fruit species are often limited, despite their value in underpinning the in-depth study of metabolic pathways, fruit ripening as well as in the identification of genotypes rich in bioactive compounds. Results: To access the genetic diversity of wild and cultivated berry fruit species that accumulate high levels of phenolic compounds in their fleshy berry(-like) fruits, we selected 13 species from Europe, South America and Asia representing eight genera, seven families and seven orders within three clades of the kingdom Plantae. RNA from either ripe fruits (ten species) or three ripening stages (two species) as well as leaf RNA (one species) were used to construct, assemble and analyse de novo transcriptomes. The transcriptome sequences are deposited in the BacHBerryGEN database (http://jicbio.nbi.ac.uk/berries) and were used, as a proof of concept, via its BLAST portal (http://jicbio.nbi.ac.uk/berries/blast.html) to identify candidate genes involved in the biosynthesis of phenylpropanoid compounds. Genes encoding regulatory proteins of the anthocyanin biosynthetic pathway (MYB and basic helix-loop-helix (bHLH) transcription factors and WD40 repeat proteins) were isolated using the transcriptomic resources of wild blackberry (Rubus genevieri) and cultivated red raspberry (Rubus idaeus cv. Prestige) and were shown to activate anthocyanin synthesis in Nicotiana benthamiana. Expression patterns of candidate flavonoid gene transcripts were also studied across three fruit developmental stages via the BacHBerryEXP gene expression browser (http://www.bachberryexp.com) in R. genevieri and R. idaeus cv. Prestige. Conclusions: We report a transcriptome resource that includes data for a wide range of berry(-like) fruit species that has been developed for gene identification and functional analysis to assist in berry fruit improvement. These resources will enable investigations of metabolic processes in berries beyond the phenylpropanoid biosynthetic pathway analysed in this study. The RNA-seq data will be useful for studies of berry fruit development and to select wild plant species useful for plant breeding purposes. ; publishersversion ; published
In: Dudnik , A , Almeida , A F , Andrade , R , Avila , B , Bañados , P , Barbay , D , Bassard , J E , Benkoulouche , M , Bott , M , Braga , A , Breitel , D , Brennan , R , Bulteau , L , Chanforan , C , Costa , I , Costa , R S , Doostmohammadi , M , Faria , N , Feng , C , Fernandes , A , Ferreira , P , Ferro , R , Foito , A , Freitag , S , Garcia , G , Gaspar , P , Godinho-Pereira , J , Hamberger , B , Hartmann , A , Heider , H , Jardim , C , Julien-Laferriere , A , Kallscheuer , N , Kerbe , W , Kuipers , O P , Li , S , Love , N , Marchetti-Spaccamela , A , Marienhagen , J , Martin , C , Mary , A , Mazurek , V , Meinhart , C , Sevillano , D M , Menezes , R , Naesby , M , Nørholm , M H H , Okkels , F T , Oliveira , J , Ottens , M , Parrot , D , Pei , L , Rocha , I , Rosado-Ramos , R , Rousseau , C , Sagot , M F , dos Santos , C N , Schmidt , M , Shelenga , T , Shepherd , L , Silva , A R , da Silva , M H , Simon , O , Stahlhut , S G , Solopova , A , Sorokin , A , Stewart , D , Stougie , L , Su , S , Thole , V , Tikhonova , O , Trick , M , Vain , P , Veríssimo , A , Vila-Santa , A , Vinga , S , Vogt , M , Wang , L , Wang , L , Wei , W , Youssef , S , Neves , A R & Forster , J 2018 , ' BacHBerry : BACterial Hosts for production of Bioactive phenolics from bERRY fruits ' , Phytochemistry Reviews , vol. 17 , no. 2 , pp. 291-326 . https://doi.org/10.1007/s11101-017-9532-2
BACterial Hosts for production of Bioactive phenolics from bERRY fruits (BacHBerry) was a 3-year project funded by the Seventh Framework Programme (FP7) of the European Union that ran between November 2013 and October 2016. The overall aim of the project was to establish a sustainable and economically-feasible strategy for the production of novel high-value phenolic compounds isolated from berry fruits using bacterial platforms. The project aimed at covering all stages of the discovery and pre-commercialization process, including berry collection, screening and characterization of their bioactive components, identification and functional characterization of the corresponding biosynthetic pathways, and construction of Gram-positive bacterial cell factories producing phenolic compounds. Further activities included optimization of polyphenol extraction methods from bacterial cultures, scale-up of production by fermentation up to pilot scale, as well as societal and economic analyses of the processes. This review article summarizes some of the key findings obtained throughout the duration of the project.
BACterial Hosts for production of Bioactive phenolics from bERRY fruits (BacHBerry) was a 3-year project funded by the Seventh Framework Programme (FP7) of the European Union that ran between November 2013 and October 2016. The overall aim of the project was to establish a sustainable and economically-feasible strategy for the production of novel high-value phenolic compounds isolated from berry fruits using bacterial platforms. The project aimed at covering all stages of the discovery and pre-commercialization process, including berry collection, screening and characterization of their bioactive components, identification and functional characterization of the corresponding biosynthetic pathways, and construction of Gram-positive bacterial cell factories producing phenolic compounds. Further activities included optimization of polyphenol extraction methods from bacterial cultures, scale-up of production by fermentation up to pilot scale, as well as societal and economic analyses of the processes. This review article summarizes some of the key findings obtained throughout the duration of the project.