Suchergebnisse

Caused by Salmonella pullorum, pullorosis is a bacterial disease threatening the poultry industry and has been listed as the bacterial disease to be eliminated by the government. However, antibiotic treatment of pullorosis has become increasingly difficult, resulting in severe influences on the sustainable development of poultry. Abuse of antibiotics may cause global drug-resistant problems. Hence, early diagnosis of young chickens and accurate treatment of sick chickens are urgently needed. Traditional serotyping for Salmonella detection is costly and labor-intensive, whereas other commonly used plate agglutination test methods often cause physical injury of chickens. Therefore, a rapid and nondamaging detection method is of great significance for early diagnosis, which is the key step in accurate medication and elimination of pullorosis. In this study, we propose a novel lateral flow nucleic acid assay (LFNAA) system combining recombinase polymerase amplification (RPA) for the detection of S. pullorum. In this method, the DNA of S. pullorum strains was quickly amplified by RPA under 37°C, and then, the RPA products were added onto the LFNAA sample pad until the final results could be observed by naked eyes within 3 min. The proposed assay is fast and delivers visible results to naked eyes in field test. The limit of detection for genomic DNA was 5 × 10(−3) ng/μL, indicating high sensitivity. In addition, the proposed LFNAA system is cost-effective, efficient, and nondamaging to chicks in the field test. This system provides technical support for early diagnosis of S. pullorum in the poultry and paves a way for future precision medicine to avoid the global drug-resistance issues.

Not my type is the usual invocation when rejecting potential lovers who don’t align with the racial hierarchy of mating preferences. The largely unchallenged norm of interracial intimacy aversion, particularly how the desire for some racial groups and rejection of others reinforces existing racial inequities, is inconsistent with the blanket notion of greater interracial acceptance. Our investigation assessed the openness of monoracial and multiracial individuals to form interracial romantic relationships. We partially replicated an interracial mate preference known as the Multiracial Dividend Effect, finding that most monoracial groups equally preferred same-race lovers and interracially dating multiracials, and they preferred interracially dating someone multiracial over any monoracial group, whereas Multiracials were more open to interracially dating any monoracial group than monoracials were to interracially date each other. In addition, Hispanic-White and East Asian-White multiracials were more open to interracially dating White individuals than their respective monoracial in-group members, and East Asian-White multiracials were more open to interracially dating all monoracial minority groups than monoracial East Asian participants. Finally, half-White multiracials are more likely to be in partial-racial couples (e.g., former President of the United States Barack Obama is Black-White multiracial and the former First Lady of the United States, Michelle Obama, is Black) whereas interminority multiracials are more likely to be in 100% interracial/non-overlapping couples (e.g., Vice President of the United States Kamala Harris is interminority Tamil Indian and Black whereas the Second Gentleman of the United States, Doug Emhoff, is White).