Suchergebnisse

WOS: 000289738800010 ; PubMed: 21255376 ; We developed a biocompatible and highly efficient approach for functionalization of bacterial cell wall with magnetic nanoparticles (MNPs). Three Acinetobacter baylyi ADP1 chromosomally based bioreporters, which were genetically engineered to express bioluminescence in response to salicylate, toluene/xylene and alkanes, were functionalized with 18 +/- 3 nm iron oxide MNPs to acquire magnetic function. The efficiency of MNPs functionalization of Acinetobacter bioreporters was 99.96 +/- 0.01%. The MNPs-functionalized bioreporters (MFBs) can be remotely controlled and collected by an external magnetic field. The MFBs were all viable and functional as good as the native cells in terms of sensitivity, specificity and quantitative response. More importantly, we demonstrated that salicylate sensing MFBs can be applied to sediments and garden soils, and semi-quantitatively detect salicylate in those samples by discriminably recovering MFBs with a permanent magnet. The magnetically functionalized cells are especially useful to complex environments in which the indigenous cells, particles and impurities may interfere with direct measurement of bioreporter cells and conventional filtration is not applicable to distinguish and harvest bioreporters. The approach described here provides a powerful tool to remotely control and selectively manipulate MNPs-functionalized cells in water and soils. It would have a potential in the application of environmental microbiology, such as bioremediation enhancement and environment monitoring and assessment. ; EPSRCEngineering & Physical Sciences Research Council (EPSRC) [EP/H04986X/1]; China National Natural Science FoundationNational Natural Science Foundation of China [40730738]; Government of the Republic of Tatarstan; Engineering and Physical Sciences Research CouncilEngineering & Physical Sciences Research Council (EPSRC) [EP/H04986X/1]; Natural Environment Research CouncilNERC Natural Environment Research Council [NE/F011938/1, CEH010021] ; We thank EPSRC Grant EP/H04986X/1 for support. We also thank China National Natural Science Foundation (Project 40730738) for financial support. The support from the Government of the Republic of Tatarstan is acknowledged by R. F. F. Authors wish to thank Mrs Ann Lowry for TEM images and EDX spectra and Mr Tony Sinclair for SEM images (University of Hull Microscopy suite).