Suchergebnisse

This study aims at evaluating the impact of different processes-pulsed electric fields (PEF), pressurized liquid extraction (PLE) and a multistep process combining PEF + PLE on the yield of antioxidant compounds (protein, polyphenols, chlorophyll a, chlorophyll b, and carotenoids) from Spirulina. Firstly, the effects of PEF or PLE treatment on the extraction yield of Spirulina biomolecules were evaluated. To further increase the extraction yield, PEF + PLE was used, as an innovative extraction approach. The results showed that PEF + PLE greatly improved the extraction yield compared with the PEF or PLE treatments alone. Compared with Folch extraction (conventional control technique), PEF + PLE significantly (P < 0.05) shortened the extraction time (−165 min) and increased the protein, polyphenol, chlorophyll a and antioxidant capacity values of Spirulina extracts by 1328%, 979%, 11% and 47% respectively. Furthermore, Triple TOF-LC-MS-MS results showed that PEF + PLE increased both the type and content of phenolic compounds. The above results were attributed to PEF-induced damage on Spirulina helical structure, which was verified by fluorescence and scanning electron microscopy. ; This research was funded by the University of Valencia through the projects OTR2021-21736INVES and OTR2021-21570INVES supported by the University of Vigo. Moreover, it was partially funded by the EU Commission and BBI-JU Horizon H2020, through the AQUABIOPRO-FIT project (aquaculture and agriculture biomass side stream proteins and bioactives for feed, fitness and health promoting nutritional supplements) grant number 790956. Jianjun Zhou and Min Wang were supported by a PhD fellowship from the China Scholarship Council (CSC) (No. 201908420246 and No. 201908420245, respectively). Authors would also like to acknowledge Generalitat Valenciana for the financial support (IDIFEDER/2018/046—Procesos innovadores de extracción y conservación: pulsos eléctricos y fluidos supercríticos) through the European Union ERDF funds (European Regional Development Fund). ; Peer reviewed

Prestellar cores are self-gravitating dense and cold structures within molecular clouds where future stars are born. They are expected, at the stage of transitioning to the protostellar phase, to harbor centrally concentrated dense (sub)structures that will seed the formation of a new star or the binary/multiple stellar systems. Characterizing this critical stage of evolution is key to our understanding of star formation. In this work, we report the detection of high-density (sub)structures on the thousand-astronomical-unit (au) scale in a sample of dense prestellar cores. Through our recent ALMA observations toward the Orion Planck Galactic Cold Clumps, we have found five extremely dense prestellar cores, which have centrally concentrated regions of similar to 2000 au in size, and several 10(7) cm(-3) in average density. Masses of these centrally dense regions are in the range of 0.30 to 6.89 M. For the first time, our higher resolution observations (0.8 '' similar to 320 au) further reveal that one of the cores shows clear signatures of fragmentation; such individual substructures/fragments have sizes of 800-1700 au, masses of 0.08 to 0.84 M, densities of 2 - 8 x 10(7) cm(-3), and separations of similar to 1200 au. The substructures are massive enough (greater than or similar to 0.1 M) to form young stellar objects and are likely examples of the earliest stage of stellar embryos that can lead to widely (similar to 1200 au) separated multiple systems. ; Ministry of Science and Technology, China 108-2112-M-001-048- 108-2112-M-001-052- international partnership program of the Chinese Academy of Sciences 114231KYSB20200009 National Natural Science Foundation of China (NSFC) 12073061 Shanghai Pujiang Program 20PJ1415500 ANID AFB 170002 AFB-170002 National Natural Science Foundation of China (NSFC) U1631237 NRC Canada Natural Sciences and Engineering Research Council of Canada (NSERC) Spanish Government AYA2017-88754-P State Agency for Research of the Spanish Ministry of Science and Innovation through the "Unit of Excellence Maria de Maeztu 2020-2023" award CEX2019-000918-M Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology NRF-2019R1A2C1010851 National Science Foundation (NSF) AST-1715876 National Natural Science Foundation of China (NSFC) 11911530226 11725313 11873086 Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT) Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research (KAKENHI) 20H05645 Yunnan Province of China 2017HC018 Chinese Academy of Sciences MoST 108-2112-M-001-017 MoST 109-2112-M-001-023 ; Versión publicada - versión final del editor