Suchergebnisse

Background : The result of attainment of performance is not quit of an applied by policy in a institution of is including also in this case hospital. Making of policy is an process which is ussul in every system. System policy maker in area treatment of RSJD Dr. RM. Soejarwadi Klaten structurally and formal is the Nursing Supervisor, arranging and controling activity of nurse in order to treatment duty execution. But that way policy which ought to be released by Nursing Supervisor isn't applicable fully yet. The mentioned cause disappearance between nurse expectation with applied policy, cause many factor influencing to intake of policy.Target of this Research is : for knowing the relation between the satisfaction of nurse with role of Nursing Supervisor in decision making in RSJD Dr. RM. Soedjarwadi Klaten.Method Research : this research type is the non eksperimental with taken sampel cross sectional method in this research is all nurse which still active work and noted as officer in the RSJD Dr. RM Soedjarwadi Klaten ( total population) : 86 responder. Data analysis for the verification of hypothesis use chi-square with alpha 0,05. Result of Research: showing p-value equal to 0,011. The value is compared to smaller of alpha 0,05, thereby can be pulled by conclusion that hypothesizing zero refused and research hypothesizing ( Ha) accepted.Conclusion : " there is a relation between the satisfaction of nurse with Role of Nursing Supervisor in decision making in RSJD Dr. R.M. Soedjarwadi Klaten".

Research of molecular characterization pfcrt gene to detection chloroquine antimalarial drugs resistance at clinic isolates Plasmodium falci parum, aims to prove and lay open mechanism and cause antimalarial drugs resistance at local clinic isolat of Plasmodium falcifarum through determination of pfmdrl gene. M ethod of the research: Making thin and thick blood films from malaria patient for microscopic inspection to determine density and identify parasite . In vitro culture to get Plasmodium falci parum parasite Isolate. Drug susceptibility testing to determine minimum inhibiting concentration (MIC) to antimalarial drugs. DNA extraction . Amplification pfcrt, gene with PCR technique. Result of research sensitivity test by in vitro Chloroquine 500 ng!ml fo r the Plasmodium fa lcipa rum isolates from malaria patient used micro test method modifyfrom WHO by using flat bottom well 96 (WHO, 1990) Result of IC50 test got Chloroquine drug can only be pursued with big dose 500 nglml. Result of PCR by using specific primary for the amplification gene region coding Pfcrt gen at 479- 630 bp genes Plasmodiumfalcifarum can be expressed so that result of this can be used as marker for the detection anti malarial drugs resistance. The concluded: M inimum concentration to pu rsue growth of parasite equal to 50%. Chloroquine require big cosentrasition to pursue growth parasite 500ng!ml. Pfcrt gene Plasmodium falcifarum can be used as marker to detect chloroquine antimalarial drugs resitance. Suggestion: Result of gene from PCR have to be continued with squencing to know mutation gene. Furthermore gene marker to make diagnostic kit as rapid test so that can give input and benefit to government evaluating and updating antimalarial drugs treatment p olicies, and give input to doctor in therapy with correct antimalarial drugs to malarial pati ents. Key words: chloroquine, resistance, pfcrt gene