Suchergebnisse

Stvaralaštvo se uvijek povezuje sa slobodnom ljudskom djelatnošću, s ljepotom, umjetnošću, ljudskom invencijom, itd. – ona je najčešće nešto prihvatljivo i dobro. Ali što se događa u vrijeme globalne manipulacije? Razvija li ovaj period nove tipove i forme stvaralaštva? Što će nam pokazati analiza rada globalne medijske manipulacije? Ideja članka je ukazati na tamnu stranu stvaralaštva u masovnim medijima. Masovni mediji smanjuju mogućnost kreativne aktivnosti u medijima dok istodobno razvijaju manipulativne oblike stvaralaštva. Ti oblici zahtijevaju da ih se ustanovi, objasni i komentira. ; Creativity is always associated with free human activity – with beauty, art, human inventions Most of the time it is something acceptable and good. But what happens in a time of global manipulation? Does the new time develop new types and forms of creativity? What will an analyses of global mass media manipulation performance reveal? The goal of the paper is to show a dark side of creativity in mass media. Mass media reduce the possibility of creative action in the media whilst they develop manipulative forms of creativity. These forms ought to be detected, explained, and commented. ; La créativité est toujours associée à l'activité libre de l'humain, à la beauté, à l'art, aux inventions humaines, etc. – elle est considérée la plupart du temps comme quelque chose d'acceptable et de bon. Or, que se passe-t-il en ces temps de manipulation globale ? Cette période développe-t-elle de nouveaux types et de nouvelles formes de créativité ? Que vont montrer les analyses sur les manipulations médiatiques auxquelles se sont livrés les acteurs mondiaux de l'univers des médias ? L'idée sous-jacente de cet article est de montrer le côté sombre de la créativité dans les médias de masse. Ces derniers réduisent la possibilité d'une action créative au sein des médias et développent en parallèle des formes manipulatoires concernant la créativité. Ces formes requièrent une identification, une explication et un commentaire. ; Die Kreativität wird stets mit der freien menschlichen Aktivität, mit der Schönheit, Kunst, den menschlichen Erfindungen usw. assoziiert – größtenteils mit etwas Akzeptablem und Gutem. Was geschieht aber in einer Zeit der globalen Manipulation? Entwickelt diese Periode neue Typen und Formen der Kreativität? Was werden uns die Analysen der Beobachtung der Medienmanipulation durch globale Spieler der Medienindustrie zeigen? Die Idee hinter dem Artikel ist es, die Schattenseiten der Kreativität in den Massenmedien aufzuzeigen. Die Massenmedien reduzieren die Möglichkeit des kreativen Handelns in den Medien. Zugleich entwickeln sie manipulative Formen der Kreativität. Diese Formen erfordern Feststellung, Erklärung und Kommentierung.

Research background. The presence of insect fragments is one of the major constrains in stored food commodities and it causes considerable loss in the quality of the produce. The management of the pest is viewed as a huge challenge in foodprocessingindustry. Conventionally, the detection of Tribolium castenaum in the food processing industry is carried out by acid hydrolysis and staining methods that are time consuming and lack precision. Experimental approach. Considering the importance of a quick and effective method, a quantitative polymerase chain reaction (qPCR)-based approach was developed and elucidated in this study. The mitochondrial cytochrome oxidase I (mtCOI) gene was identified as a target due to its abundance in the pest. Specific primers were designed against the target gene by Primer Premier software and amplified in a qPCR. Results and conclusions. This method is capable of detecting all the ontogenic stages of T. castaneum in stored wheat flour. Earlier experiments had demonstrated that about 20 µg of DNA can be obtained from 2.2 mg of insects. To quantify the infestation levels, the cycle threshold (Ct) values obtained from known samples were subjected to regression analysis and expressed as adult equivalents. In the unknown samples, the infestation was calculated as 1.74 and 0.046 adult insects in 5 g of wheat flour. The maximum permissible limit of insect fragments in flour is 75 insect fragments or approx. 3 adults per 50 g of flour as per the US Food and Drug Administration (FDA). Hence, by adopting this new method, it is possible for the warehouse operators to arrive at a decision to proceed with efficient management practices where wheat flour is stored. Also, this method can be ratified by government agencies associated with international business to ascertain whether the wheat flour meets the standards set by the respective country before subjecting to foreign trade. Novelty and scientific contribution. This study is the first of its kind in the detection and quantification of T. castaneum in milled products. So far, only conventional methods have been employed to assess the presence of the pests and manual counting of fragments are practiced to quantify the infestation levels. The developed qPCR method is faster, reliable and can be employed in milling industries, bakery industries, food processing plants and foreign trade units for critical detection and quantification of T. castaneum pest infestation. ; Pozadina istraživanja. Prisutnost dijelova insekata u namirnicama jedno je od glavnih ograničenja pri njihovom skladištenju, te uzrokuje velike gubitke u kvaliteti proizvoda. Suzbijanje štetnika predstavlja velik izazov u prehrambenoj industriji. Tradicionalno se prisutnost kestenjastog brašnara (Tribolium castaneum) u prehrambenim proizvodima utvrđuje kiselinskom hidrolizom i metodom bojanja, koje su dugotrajne i neprecizne. Eksperimentalni pristup. Uzevši u obzir potrebu za primjenom brze i učinkovite metode određivanja prisutnosti T. castaeneum u brašnu, u radu je razvijena i opisana kvantitativna metoda koja se temelji na lančanoj reakciji polimerazom u stvarnom vremenu (qRT-PCR). Kao ciljni gen odabran je mitohondrijski gen za podjedinicu 1 citokrom c oksidaze (mtCOI) zbog njegove rasprostranjenosti u nametniku. Pomoću kompjuterskog softvera Primer Premier izrađene su početnice za umnožavanje DNA ciljane vrste metodom qRT-PCR. Rezultati i zaključci. Ovom se metodom može identificirati kestenjasti brašnar (T. castaneum) u svim fazama ontogeneze u uskladištenom brašnu. Prijašnji su eksperimenti pokazali da se iz 2,2 mg kukaca može izdvojiti otprilike 20 µg DNA. Za utvrđivanje opsega zaraze provedena je regresijska analiza broja ciklusa (Ct-vrijednost) potrebnih za određivanje poznatih uzoraka, a rezultati su izraženi kao broj odraslih kukaca. U nepoznatim je uzorcima izračunat stupanj zaraze od 1,74 odnosno 0,046 odraslih insekata u 5 g pšeničnog brašna. Prema regulativi Američke agencije za hranu i lijekove (engl. U.S. Food and Drug Administration (FDA)), najveći dopušteni broj dijelova insekata u brašnu je 75, odnosno otprilike 3 odrasla kukca u 50 kg brašna. Pomoću ove nove metode voditelji skladišta mogu donijeti odluku o tome gdje skladištiti brašno, te učinkovito provoditi plan suzbijanja ovog nametnika. Također, državne agencije koje posreduju u međunarodnom poslovanju mogu temeljem ove metode utvrditi zadovoljava li kakvoća proizvedenog pšeničnog brašna standarde zemalja kojima se ono isporučuje. Novina i znanstveni doprinos. Ovo je prvo istraživanje u kojem je utvrđena količina kestenjastog brašnara T. castaneum u mljevenim žitaricama. Dosad su korištene samo konvencionalne metode utvrđivanja prisustva nametnika u brašnu, a opseg zaraze je određivan ručnim brojanjem dijelova insekata. Nova qRT-PCR metoda je brža, pouzdanija i može se koristiti u mlinarskoj i pekarskoj industriji, pogonima za preradu hrane i pri međunarodnoj trgovini za utvrđivanje opsega zaraze kestenjastim brašnarom.

U ovom radu, u razmatranju povijesnog razvoja stanovništva u zapadnoeuropskim zemljama u razdoblju nakon sredine 1960-ih godina, težište je izlaganja na temi "druga demografska tranzicija." Analizirat ćemo demografska i socio-demografska obilježja toga razdoblja, sličnosti i razlike s etapom prethodne demografske tranzicije te podudarnost "druge demografske tranzicije" s posttranzicijskom etapom u razvoju stanovništva. Nastojat ćemo odgovoriti na temeljno pitanje koje se u tim razmatranjima postavlja: može li se na osnovi empirijske i znanstvene analize "druga demografska tranzicija" smatrati "posebnom, novom etapom u razvoju stanovništva" ili je ona samo drugo ime za posttranzicijsku etapu kao etapu nakon prethodne ("prve") demografske tranzicije. U novim razvojnim uvjetima i uz nove ekonomskosocijalne, tehničko-tehnološke, vrijednosne, kulturološke i socio-psihološke čimbenike, "druga demografska tranzicija" predstavlja kontinuitet u odnosu na ustanovljen trend smanjivanja nataliteta/fertiliteta u prethodnoj demografskoj tranzicij i, koji je u novim uvjetima života i rada te djelovanju novih, napose vrijednosnih čimbenika relevantnih za postindustrijsko i postmodernizacijsko društvo, usmjeren na postignuće generacijski ispodzamjenskog nataliteta/fertiliteta. ; The second demographic transition came about in European demographic literature as a topic in 1986. The authors thereof were Dutch demographers Dirk van de Kaa and Ron Lesthaeghe. Starting from the demographic-historical conceptualisation of the theory of demographic transition, it may be concluded that following the fi rst demographic transition, the stage the authors call the second demographic transition occured in the development of the population. This is however only another name for the post-transitional stage, whereby this term includes the continuity of demographic trends, while the term the second demographic transition explains the discontinuity between the periods of the fi rst and the second demographic transition. Van de Kaa (1987) emphasises that due to the impact of essential specifi c factors, essential diff erences emerged between these two transitions. During the second demographic transition, they were caused by secularisation and individualisation processes, and new factors linked with them (new value orientation; socio-psychological and other factors). They caused a decline of marriages; an increase in the number of cohabitations and other forms of life partnerships; an increase in the number of children born out of wedlock; an increase in the number of divorces; etc., which have become acceptable in the perception of the young generation. On the contrary, having children and the number of children have become a matt er of partners' free choice, as their primary goal is to achieve self-fulfi lment at personal level. It has all exercised an impact on a further fertility reduction, which started in the middle phase, and became intensifi ed in the late phase of the fi rst transition. Essential diff erences between the fi rst and the second transition, which the authors mention in the paper, have however arisen from the understandable fact that each period bear their own specifi c historical context and specifi c features of social diff erentiation. The authors point out that essential diff erences between the two transitions have further arisen from the main postulates they are based upon. The fi nal stage of the fi rst transition was based on balance (zero level) between low birth and death rates, and on stationary population. In the second demographic transition, birth and fertility rates tended to be reduced to the sub-replacement level, or, according to Lesthaeghe, to the sustainable sub-replacement fertility level, which, along with life prolongation, intensifi ed population aging, which demanded the so-called replacement migration as a compensation for the decrease in workforce. Starting from the thesis that the second demographic transition was a new stage in European demographic history, separated from the fi rst transition, the authors emphasised that there was no continuity in fertility reduction between the two transitions, as in the second transition, this trend was oriented towards the permanent and sustainable sub-replacement fertility level. However, many other demographers, such as R. Cliquet and D. Coleman, hold that between the two transitions, there was a continuity in demographic trends, in particular fertility reduction, which leaned on the low fertility level already achieved in the fi nal stage of the fi rst demographic transition. Our research has led to the identical conclusion, as it commenced from the fact that fertility reduction started in the middle phase of the fi rst transition and became intensifi ed in the late phase thereof; hence, fertility reduction in the second demographic transition continued to the balance of low levels of these rates, already achieved in the fi rst transition. The turn that occurred in the fi rst decade of the 21st century indicated to the possibility of the emergence of new factors in new social circumstances, which might infl uence the growth of birth/fertility rates. According to the available data, the total fertility rate in most of Western European countries grew and approximated the replacement level, yet did not exceed it. A longer period of studying this complex phenomenon is hence rate decrease to the sub-replacement level was irreversible and permanently sustainable in highly-developed countries.