Cellular mechanism for regulation of ion transport in human bronchial epithelial cells by Cordyceps militaris extract and its isolated compound cordycepin
Fung, Chun Kit. ; Thesis (M.Phil.)--Chinese University of Hong Kong, 2011. ; Includes bibliographical references (leaves 121-135). ; Abstracts in English and Chinese. ; Declaration --- p.i ; Acknowledgement --- p.ii ; Abbreviations --- p.iii ; Abstract in English --- p.v ; Abstract in Chinese --- p.vii ; Table of Contents --- p.ix ; List of Figures --- p.xii ; Chapter Chapter 1 - --- Introduction ; Chapter 1.1 --- Genus Cordyceps --- p.1 ; Chapter 1.2 --- Cordyceps militaris --- p.4 ; Chapter 1.3 --- Biological Functions and Chemical Constituents of Cordyceps militaris --- p.9 ; Chapter 1.4 --- "Human Bronchial Epithelial Cell Line, 16HBE14o-" --- p.13 ; Chapter 1.5 --- Ion Transport in Human Bronchial Epithelial Cells --- p.16 ; Chapter 1.6 --- Objectives of the Experiments --- p.20 ; Chapter Chapter 2 - --- Materials and Methods ; Chapter 2.1 --- Solutions and Chemicals --- p.21 ; Chapter 2.2 --- Preparation of Hot Water Cordyceps militaris Extract --- p.22 ; Chapter 2.3 --- Culture of Cells --- p.23 ; Chapter 2.4 --- Short-Circuit Current (lsc) Measurement --- p.24 ; Chapter 2.5 --- Short-Circuit Current (lsc) Measurement in Nystatin-Permeabilized Monolayer --- p.29 ; Chapter 2.6 --- Measurements of [Ca2+]i --- p.31 ; Chapter 2.7 --- Measurement of PKA Activity --- p.36 ; Chapter 2.8 --- Statistical Analysis --- p.37 ; Chapter Chapter 3 - --- Results ; Chapter 3.1 --- Regulation of Ion Transport in 16HBE14o- Cells by CM Water Extract --- p.38 ; Chapter 3.1.1 --- Dose-Dependent Relationship of CM Water Extract --- p.39 ; Chapter 3.1.2 --- "Involvement of CI"" Transport in CM-induced lsc Response" --- p.42 ; Chapter 3.1.3 --- Involvement of K+ channels in CM-induced lsc Response --- p.47 ; Chapter 3.1.4 --- Involvement of Adenylate Cyclase/cAMP/Protein Kinase A Pathway in CM-induced lsc Response --- p.52 ; Chapter 3.1.5 --- Involvement of Ca2+-Dependent Pathway in CM-induced lsc Response --- p.57 ; Chapter 3.1.6 --- "Effect of CM Extract on Apical CI"" Current and Basolateral K+ Current in ...