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Eight seafood protein hydrolysates (SPHs) obtained from squid, shrimp and fish gelatin were incorporated as substitutes of peptones in culture media in order to evaluate its effect on survival and metabolic activity (lactic acid, acetic acid and bacteriocins production) of Enterococcus faecalis DM19. The substitution of commercial peptones in culture media by either a shrimp hydrolysate prepared with Protamex, or by squid protein hydrolysates prepared with Esperase or Alkaline protease, stimulated E. faecalis DM19 growth up to 16%. The incorporation of SPHs, mainly from shrimp, in the culture media significantly increased production of lactic and acetic acids in more than 60%. Furthermore, the media containing SPHs stimulated antimicrobial activity by E. faecalis DM19. The inhibitory activity was observed against both Gram-positive and Gram-negative microorganisms, but it was remarkably observed against Listeria monocytogenes. SPHs incorporated in culture media render properties of bio-technological interest, which, together with their low price, make them suitable for industrial use. ; This research was financed by the Spanish Ministry of Economy and Competitiveness (projects AGL2011-27607, AGL2014-52825-R). Author M. Djellouli is funded by The National Centre of Biotechnology Research (CHER-Stage 06-2013) (CNRBt) of Algeria and ENP (Exceptional National Program) Scholarship provided by the Ministry of Higher Education and Scientific Research of Algeria (099/PNE/ENS./ESPAGNE/2015-2016). Author M. Arancibia is funded by a SENESCYT Scholarship (20100338) provided by the Ecuadorian government. ; Peer Reviewed