author:"Akhlamionok, T. P." | Pollux - Fachinformationsdienst Politikwissenschaft

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Article(electronic)#1November 29, 2022

Optimization of the technology for obtaining guide RNA using plate automatic synthesizer

In: Proceedings of the National Academy of Sciences of Belarus, Chemical Series, Volume 58, Issue 4, p. 398-406

Ulashchik, E. A.; Akhlamionok, T. P.; Baryshchyk, P. Y.; Sharko, O. L.; Shmanai, V. V.

ISSN: 2524-2342

A synthetic protocol was developed and optimal reagents have been selected for obtaining guide RNA oligonucleotides for the CRISPR/Cas system using ASM-2000 automatic synthesizer in 500 nmol scale. Methods for the isolation, purification and analytical control of synthetic RNA oligonucleotides have been developed. The improved technology has been used for preparation of guide RNAs for the CRISPR Cas12a system.

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Article(electronic)#2March 4, 2024

Synthesis of thiol-containing DNA-oligonucleotides using a phosphoramidite reagent based on trans -4-hydroxy-L-prolinol

In: Proceedings of the National Academy of Sciences of Belarus, Chemical Series, Volume 60, Issue 1, p. 36-44

Lamekina, Y. P.; Seviarynchyk, T. P.; Ulashchik, E. A.; Akhlamionok, T. P.; Baryshchyk, P. Y.; Ranishenka, B. V.; Shmanai, V. V.