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[EN] In recent years nanotechnology has become a significant component in food industry. It is present in all food chain steps, from the design of new ingredients or additives, to the most modern systems of food quality methods or packaging, demonstrating the great potential of this new technology in a sector as traditional as food. However, while interest by industry in nanotechnology increases, the rejection by consumers, concerned about the potential risk, does too. The aim of this review is to evaluate the development of food nanotechnology by means of a patent analysis, highlighting current applications of nanotechnology along the whole food chain and contextualizing this evolution in the social scene. ; Financial support from the Spanish Government (project MAT2009-14564-C04-01 and AGL2010-20539) and the Generalitat Valencia (project PROMETEO/2009/016) are gratefully acknowledged. E.P. thanks the Ministerio de Educación for a fellowship ; Pérez-Esteve, É.; Bernardos Bau, A.; Martínez-Máñez, R.; Barat Baviera, JM. (2013). Nanotechnology in the Development of Novel Functional Foods or their Package. An Overview Based in Patent Analysis. Recent Patents on Food, Nutrition and Agriculture. 5(1):35-43. https://doi.org/10.2174/2212798411305010006 ; S ; 35 ; 43 ; 5 ; 1

[EN] This review summarises examples of capped mesoporous silica materials for controlled delivery that use enzymes as external triggers or functional components of the gating ensemble. ; Financial support from the Spanish Government and FEDER funds (Project MAT2015-64139-C4-1) and the Generalitat Valencia (Project PROMETEOII/2014/047) is gratefully acknowledged. A. Llopis-Lorente is grateful to "La Caixa'' Banking Foundation for his PhD fellowship. B. Lozano-Torres is grateful to the Spanish Ministry of Economy and Competitiveness (MEC) for her FPU grant. Also A. Bernardos thanks the Spanish MEC for her Juan de la Cierva Contract. ; Llopis-Lorente, A.; Lozano-Torres, B.; Bernardos Bau, A.; Martínez-Máñez, R.; Sancenón Galarza, F. (2017). Mesoporous silica materials for controlled delivery based on enzymes. Journal of Materials Chemistry B. 5(17):3069-3083. https://doi.org/10.1039/C7TB00348J ; S ; 3069 ; 3083 ; 5 ; 17

3069 3083 5 17 ; S ; [EN] This review summarises examples of capped mesoporous silica materials for controlled delivery that use enzymes as external triggers or functional components of the gating ensemble. Financial support from the Spanish Government and FEDER funds (Project MAT2015-64139-C4-1) and the Generalitat Valencia (Project PROMETEOII/2014/047) is gratefully acknowledged. A. Llopis-Lorente is grateful to "La Caixa'' Banking Foundation for his PhD fellowship. B. Lozano-Torres is grateful to the Spanish Ministry of Economy and Competitiveness (MEC) for her FPU grant. Also A. Bernardos thanks the Spanish MEC for her Juan de la Cierva Contract. Llopis-Lorente, A.; Lozano-Torres, B.; Bernardos Bau, A.; Martínez-Máñez, R.; Sancenón Galarza, F. (2017). Mesoporous silica materials for controlled delivery based on enzymes. Journal of Materials Chemistry B. 5(17):3069-3083. https://doi.org/10.1039/C7TB00348J

This is the peer reviewed version of the following article: Bernardos, A., Piacenza, E., Sancenón, F., Hamidi, M., Maleki, A., Turner, R. J., Martínez-Máñez, R., Mesoporous Silica-Based Materials with Bactericidal Properties. Small 2019, 15, 1900669. https://doi.org/10.1002/smll.201900669 , which has been published in final form at https://doi.org/10.1002/smll.201900669. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. ; [EN] Bacterial infections are the main cause of chronic infections and even mortality. In fact, due to extensive use of antibiotics and, then, emergence of antibiotic resistance, treatment of such infections by conventional antibiotics has become a major concern worldwide. One of the promising strategies to treat infection diseases is the use of nanomaterials. Among them, mesoporous silica materials (MSMs) have attracted burgeoning attention due to high surface area, tunable pore/particle size, and easy surface functionalization. This review discusses how one can exploit capacities of MSMs to design and fabricate multifunctional/controllable drug delivery systems (DDSs) to combat bacterial infections. At first, the emergency of bacterial and biofilm resistance toward conventional antimicrobials is described and then how nanoparticles exert their toxic effects upon pathogenic cells is discussed. Next, the main aspects of MSMs (e.g., physicochemical properties, multifunctionality, and biosafety) which one should consider in the design of MSM-based DDSs against bacterial infections are introduced. Finally, a comprehensive analysis of all the papers published dealing with the use of MSMs for delivery of antibacterial chemicals (antimicrobial agents functionalized/adsorbed on mesoporous silica (MS), MS-loaded with antimicrobial agents, gated MS-loaded with antimicrobial agents, MS with metal-based nanoparticles, and MS-loaded with metal ions) is provided. ; The authors thank the Spanish Government (projects MAT2015-64139-C4-1-R and ...

[EN] In recent decades, the versatility of mesoporous silica particles and their relevance to develop controlled release systems have been demonstrated. Within them, gated materials able to modulate payload delivery represent great advantages. However, the role played by the porous matrix in this kind of systems is scarce. In this work, different mesoporous silica materials (MCM-41, MCM-48, SBA-15 and UVM-7) are functionalized with oleic acid as a molecular gate. All systems are fully characterized and their ability to confine the entrapped cargo and release it in the presence of bile salts is validated with release assays and in vitro digestion experiments. The cargo release profile of each synthesized support is studied, paying attention to the inorganic scaffold. Obtained release profiles fit to Korsmeyer-Peppas model, which explains the differences among the studied supports. Based on the results, UVM-7 material was the most appropriate system for duodenal delivery and was tested in an in vivo model of the Wistar rat. Payload confinement and its complete release after gastric emptying is achieved, establishing the possible use of mesoporous silica particles as protection and direct release agents into the duodenum and, hence, demonstrating that these systems could serve as an alternative to the administration methods employed until now. ; This research was funded by the Spanish Government (projects RTI2018-100910-B-C41 and RTI2018-101599-B-C22-AR (MCUI/FEDER, EU)) and the Generalitat Valenciana (project PROMETEO/2018/024 and ACIF/2016/023 grant). ; Poyatos-Racionero, E.; González-Álvarez, I.; González-Álvarez, M.; Martínez-Máñez, R.; Marcos Martínez, MD.; Bernardos Bau, A.; Aznar, E. (2020). Surfactant-Triggered Molecular Gate Tested on Different Mesoporous Silica Supports for Gastrointestinal Controlled Delivery. Nanomaterials. 10(7):1-18. https://doi.org/10.3390/nano10071290 ; S ; 1 ; 18 ; 10 ; 7 ; Pérez-Esteve, É., Ruiz-Rico, M., de la Torre, C., Llorca, E., Sancenón, F., Marcos, M. D., … Barat, J. M. ...

Essential oil components (EOCs) are known for their antifungal properties; however, their high volatility limits their application as antimicrobial agents. Strategies used for controlling the volatility of EOCs include encapsulation or loading into porous materials. This study evaluated the in vitro antifungal activity of selected EOCs (carvacrol, cinnamaldehyde, eugenol and thymol) against the fungus Aspergillus niger when loaded into MCM-41 and b-cyclodextrin (b-CD). RESULTS: Carvacrol and thymol in Mobil Composition of Matter No. 41 (MCM-41) displayed remarkable enhanced antifungal properties in comparison to the pure or b- CD-encapsulated EOCs. In fact, carvacrol and thymol were able to maintain antifungal activity and inhibit fungal growth for 30 days, suggesting better applicability of these EOCs as natural preservatives. CONCLUSIONS: The sustained antifungal effect of EOCs encapsulated into silica mesoporous supports was described. ; The authors wish to express their gratitude to the Government of the Czech Republic (Postdok CZU: ESF and MEYS CZ.1.07/2.3.00/30.0040), to CIGA (Project number: 20132024) and to the Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic (research project RVO: 61388963). Beth Green is thanked for language control. ; Bernardos Bau, A.; Marina, T.; Zacek, P.; Pérez-Esteve, É.; Martínez-Máñez, R.; Lhotka, M.; Kourimská, L. (2015). Antifungal effect of essential oil components against Aspergillus niger when loaded into silica mesoporous supports. Journal of the Science of Food and Agriculture. 95(14):2824-2831. https://doi.org/10.1002/jsfa.7022 ; S ; 2824 ; 2831 ; 95 ; 14

[EN] The induction of senescence produces a stable cell cycle arrest in cancer cells, thereby inhibiting tumor growth; however, the incomplete immune cell-mediated clearance of senescent cells may favor tumor relapse, limiting the long-term anti-tumorigenic effect of such drugs. A combination of senescence induction and the elimination of senescent cells may, therefore, represent an efficient means to inhibit tumor relapse. In this study, we explored the antitumor efficacy of a combinatory senogenic and targeted senolytic therapy in an immunocompetent orthotopic mouse model of the aggressive triple negative breast cancer subtype. Following palbociclib-induced senogenesis and senolysis by treatment with nano-encapsulated senolytic agent navitoclax, we observed inhibited tumor growth, reduced metastases, and a reduction in the systemic toxicity of navitoclax. We believe that this combination treatment approach may have relevance to other senescence-inducing chemotherapeutic drugs and additional tumor types. Significance: While the application of senescence inducers represents a successful treatment strategy in breast cancer patients, some patients still relapse, perhaps due to the subsequent accumulation of senescent cells in the body that can promote tumor recurrence. We now demonstrate that a combination treatment of a senescence inducer and a senolytic nanoparticle selectively eliminates senescent cells, delays tumor growth, and reduces metastases in a mouse model of aggressive breast cancer. Collectively, our results support targeted senolysis as a new therapeutic opportunity to improve outcomes in breast cancer patients. ; The M.O. laboratory members thank the financial support from the Spanish Government (project SAF2017-84689-R (MINECO/AEI/FEDER, EU)) and the Generalitat Valenciana (project PROMETEO/2019/065). The R.M. laboratory members thank the financial support from the Spanish Government (projects RTI2018-100910-B-C41 and RTI2018-101599-B-C22 (MCUI/FEDER, EU) and the Generalitat Valenciana (project ...

[EN] A new delivery microdevice, based on hydrophobic oleic acid¿capped mesoporous silica particles and able to payload release in the presence of surfactants, has been developed. The oleic acid functionalization confers to the system a high hydrophobic character, which avoids cargo release unless surfactant molecules are present. The performance of this oleic¿acid capped microdevice in the presence of different surfactants is presented and its zero¿release operation in the absence of surfactants is demonstrated. ; The authors want to thank the Spanish Government (projects MAT2015-64139-C4-1-R, AGL2015-70235-C2-1-R and AGL2015-70235-C2-2-R (MINECO/FEDER)) and RTI2018-100910-B-C41, RTI2018-101599-B-C22 and RTI2018-101599-B-C21 (MCUI/AEI/FEDER, UE)) and Generalitat Valenciana (project PROMETEO/2018/024) for support. E.P.-R. thanks the Generalitat Valenciana for her predoctoral fellowship. A.B. wants to acknowledge the Spanish Government for the financial support Juan de la Cierva Incorporación IJCI-2014-21534. The authors also thank the Electron Microscopy Service at the UPV for support. ; Poyatos-Racionero, E.; Pérez-Esteve, É.; Marcos Martínez, MD.; Barat Baviera, JM.; Martínez-Máñez, R.; Aznar, E.; Bernardos Bau, A. (2019). New Oleic Acid-Capped Mesoporous Silica Particles as Surfactant-Responsive Delivery Systems. ChemistryOpen. 8(8):1052-1056. https://doi.org/10.1002/open.201900092 ; S ; 1052 ; 1056 ; 8 ; 8 ; HARRISON, K. (2007). Introduction to polymeric drug delivery systems. Biomedical Polymers, 33-56. doi:10.1533/9781845693640.33 ; Bourganis, V., Karamanidou, T., Kammona, O., & Kiparissides, C. (2017). Polyelectrolyte complexes as prospective carriers for the oral delivery of protein therapeutics. European Journal of Pharmaceutics and Biopharmaceutics, 111, 44-60. doi:10.1016/j.ejpb.2016.11.005 ; El-Safty, S. A., Shenashen, M. A., & Ismail, A. A. (2012). A multi-pH-dependent, single optical mesosensor/captor design for toxic metals. Chemical Communications, 48(77), 9652. doi:10.1039/c2cc34788a ...

1052 1056 8 ; S Kickelbick, G. (2004). Mesoporöse anorganisch-organische Hybridmaterialien. Angewandte Chemie, 116(24), 3164-3166. doi:10.1002/ange.200301751 ; Candel, I., Aznar, E., Mondragón, L., Torre, C. de la, Martínez-Máñez, R., Sancenón, F., … Parra, M. (2012). Amidase-responsive controlled release of antitumoral drug into intracellular media using gluconamide-capped mesoporous silica nanoparticles. Nanoscale, 4(22), 7237. doi:10.1039/c2nr32062b ; [EN] A new delivery microdevice, based on hydrophobic oleic acid¿capped mesoporous silica particles and able to payload release in the presence of surfactants, has been developed. The oleic acid functionalization confers to the system a high hydrophobic character, which avoids cargo release unless surfactant molecules are present. The performance of this oleic¿acid capped microdevice in the presence of different surfactants is presented and its zero¿release operation in the absence of surfactants is demonstrated. The authors want to thank the Spanish Government (projects MAT2015-64139-C4-1-R, AGL2015-70235-C2-1-R and AGL2015-70235-C2-2-R (MINECO/FEDER)) and RTI2018-100910-B-C41, RTI2018-101599-B-C22 and RTI2018-101599-B-C21 (MCUI/AEI/FEDER, UE)) and Generalitat Valenciana (project PROMETEO/2018/024) for support. E.P.-R. thanks the Generalitat Valenciana for her predoctoral fellowship. A.B. wants to acknowledge the Spanish Government for the financial support Juan de la Cierva Incorporación IJCI-2014-21534. The authors also thank the Electron Microscopy Service at the UPV for support. Poyatos-Racionero, E.; Pérez-Esteve, É.; Marcos Martínez, MD.; Barat Baviera, JM.; Martínez-Máñez, R.; Aznar, E.; Bernardos Bau, A. (2019). New Oleic Acid-Capped Mesoporous Silica Particles as Surfactant-Responsive Delivery Systems. ChemistryOpen. 8(8):1052-1056. https://doi.org/10.1002/open.201900092 HARRISON, K. (2007). Introduction to polymeric drug delivery systems. Biomedical Polymers, 33-56. doi:10.1533/9781845693640.33 Bourganis, V., Karamanidou, T., Kammona, O., & ...

[EN] Organic-inorganic hybrid nanomaterials offer extremely valuable tools for monitoring many types of analytes in solution. Within this framework, aptamer-based nanomaterials for heavy metal detection are still very scarce. Herein, a novel sensing nanoprobe for the selective and sensitive detection of As(III) based on the combination of aptamers with mesoporous silica nanoparticles has been developed. The efficiency of the sensor is demonstrated in environmental conditions, showing a great potential in As(III) monitoring assays. ; The authors thank the financial support from the Spanish Government (projects MAT2015-64139-C4-1-R and AGL2015-70235-C2-2-R) and the Generalitat Valenciana (project GVA/2014/13). M.O. is grateful to the Universitat Politecnica for her PhD grant. A.B. thanks the Spanish Government for the finantial support "Juan de la Cierva-Incorporacion" (IJCI-2014-21534). C.C. thanks the Generalitat Valenciana for her postdoctoral contract VALi+D. ; Oroval, M.; Coll Merino, MC.; Bernardos Bau, A.; Marcos Martínez, MD.; Martínez-Máñez, R.; Shchukin, DG.; Sancenón Galarza, F. (2017). Selective Fluorogenic Sensing of As(III) Using Aptamer-Capped Nanomaterials. ACS Applied Materials & Interfaces. 9(13):11332-11336. https://doi.org/10.1021/acsami.6b15164 ; S ; 11332 ; 11336 ; 9 ; 13

[EN] Mesoporous silica nanoparticles loaded with fluorescein and capped by a pseudorotaxane, formed between a naphthalene derivative and cyclobis(paraquat-p-phenylene) (CBPQT(4+)), were used for the selective and sensitive fluorogenic detection of 3,4-methylenedioxy-methamphetamine (MDMA). ; The authors acknowledge the financial support from the Spanish Government (projects MAT2015-64139-C4-1-R and AGL2015-70235-C2-2-R) and the Generalitat Valenciana (project GVA/2014/13). B. L-T. is grateful to the Ministerio de Economia y Competitividad for her PhD grant. A. B. thanks the Spanish Government for the financial support "Juan de la CiervaIncorporacion'' (IJCI-2014-21534). J. O. J. thanks The Danish Natural Science Research Council (FNU, project no. 11-106744) and the Villum Foundation for financial support. ; Lozano-Torres, B.; Pascual, L.; Bernardos Bau, A.; Marcos Martínez, MD.; Jeppesen, JO.; Salinas Soler, Y.; Martínez-Máñez, R. (2017). Pseudorotaxane capped mesoporous silica nanoparticles for 3,4-methylenedioxymethamphetamine (MDMA) detection in water. Chemical Communications. 53(25):3559-3562. https://doi.org/10.1039/c7cc00186j ; S ; 3559 ; 3562 ; 53 ; 25 ; McLELLAN, A. T., LUBORSKY, L., WOODY, G. E., & O??BRIEN, C. P. (1980). An Improved Diagnostic Evaluation Instrument for Substance Abuse Patients. The Journal of Nervous and Mental Disease, 168(1), 26-33. doi:10.1097/00005053-198001000-00006 ; Moonzwe, L. S., Schensul, J. J., & Kostick, K. M. (2011). The Role of MDMA (Ecstasy) in Coping with Negative Life Situations Among Urban Young Adults. Journal of Psychoactive Drugs, 43(3), 199-210. doi:10.1080/02791072.2011.605671 ; Meyer, J. (2013). 3,4-methylenedioxymethamphetamine (MDMA): current perspectives. Substance Abuse and Rehabilitation, 83. doi:10.2147/sar.s37258 ; Greene, S. L., Kerr, F., & Braitberg, G. (2008). Review article: Amphetamines and related drugs of abuse. Emergency Medicine Australasia, 20(5), 391-402. doi:10.1111/j.1742-6723.2008.01114.x ; Cumba, L. R., Smith, J. P., Zuway, K. ...

3559 3562 53 25 ; S ; [EN] Mesoporous silica nanoparticles loaded with fluorescein and capped by a pseudorotaxane, formed between a naphthalene derivative and cyclobis(paraquat-p-phenylene) (CBPQT(4+)), were used for the selective and sensitive fluorogenic detection of 3,4-methylenedioxy-methamphetamine (MDMA). The authors acknowledge the financial support from the Spanish Government (projects MAT2015-64139-C4-1-R and AGL2015-70235-C2-2-R) and the Generalitat Valenciana (project GVA/2014/13). B. L-T. is grateful to the Ministerio de Economia y Competitividad for her PhD grant. A. B. thanks the Spanish Government for the financial support "Juan de la CiervaIncorporacion'' (IJCI-2014-21534). J. O. J. thanks The Danish Natural Science Research Council (FNU, project no. 11-106744) and the Villum Foundation for financial support. Lozano-Torres, B.; Pascual, L.; Bernardos Bau, A.; Marcos Martínez, MD.; Jeppesen, JO.; Salinas Soler, Y.; Martínez-Máñez, R. (2017). Pseudorotaxane capped mesoporous silica nanoparticles for 3,4-methylenedioxymethamphetamine (MDMA) detection in water. Chemical Communications. 53(25):3559-3562. https://doi.org/10.1039/c7cc00186j McLELLAN, A. T., LUBORSKY, L., WOODY, G. E., & O??BRIEN, C. P. (1980). An Improved Diagnostic Evaluation Instrument for Substance Abuse Patients. The Journal of Nervous and Mental Disease, 168(1), 26-33. doi:10.1097/00005053-198001000-00006 Moonzwe, L. S., Schensul, J. J., & Kostick, K. M. (2011). The Role of MDMA (Ecstasy) in Coping with Negative Life Situations Among Urban Young Adults. Journal of Psychoactive Drugs, 43(3), 199-210. doi:10.1080/02791072.2011.605671 Meyer, J. (2013). 3,4-methylenedioxymethamphetamine (MDMA): current perspectives. Substance Abuse and Rehabilitation, 83. doi:10.2147/sar.s37258 Greene, S. L., Kerr, F., & Braitberg, G. (2008). Review article: Amphetamines and related drugs of abuse. Emergency Medicine Australasia, 20(5), 391-402. doi:10.1111/j.1742-6723.2008.01114.x Cumba, L. R., Smith, J. P., Zuway, K. Y., Sutcliffe, O. B., ...

[EN] Aptamers have been used as recognition elements for several molecules due to their great affinity and selectivity. Additionally, mesoporous nanomaterials have demonstrated great potential in sensing applications. Based on these concepts, we report herein the use of two aptamer¿capped mesoporous silica materials for the selective detection of ochratoxin¿A (OTA). A specific aptamer for OTA was used to block the pores of rhodamine¿B¿loaded mesoporous silica nanoparticles. Two solids were prepared in which the aptamer capped the porous scaffolds by using a covalent or electrostatic approach. Whereas the prepared materials remained capped in water, dye delivery was selectively observed in the presence of OTA. The protocol showed excellent analytical performance in terms of sensitivity (limit of detection: 0.5¿0.05¿nm), reproducibility, and selectivity. Moreover, the aptasensors were tested for OTA detection in commercial foodstuff matrices, which demonstrated their potential applicability in real samples ; We thank the Spanish Government (projects MAT2015-64139-C4-1-R and AGL2015-70235-C2-2-R (MINECO/FEDER)) and the Generalitat Valenciana (project PROMETEOII/2014/047) for support. A.R. thanks UPV for her predoctoral fellowship. S.S. thanks the Instituto de Salud Carlos III and the European Social Fund for the financial support "Sara Borrell" (CD16/000237). A.B. thanks the Spanish Government for financial support by the "Juan de la Cierva-Incorporacion" (IJCI-2014-21534). The authors also thank the Electron Microscopy Service at UPV for support. ; Ribes, À.; Santiago Felipe, S.; Bernardos Bau, A.; Marcos Martínez, MD.; Pardo Vicente, MT.; Sancenón Galarza, F.; Martínez-Máñez, R. (2017). Two New Fluorogenic Aptasensors Based on Capped Mesoporous Silica Nanoparticles to Detect Ochratoxin A. ChemistryOpen. 6(5):653-659. https://doi.org/10.1002/open.201700106 ; S ; 653 ; 659 ; 6 ; 5

[EN] A naphthalimide-based two-photon probe (AHGa) for the detection of cell senescence is designed. The probe contains a naphthalimide core, an L-histidine methyl ester linker, and an acetylated galactose bonded to one of the aromatic nitrogen atoms of the L-histidine through a hydrolyzable N-glycosidic bond. Probe AHGa is transformed into AH in senescent cells resulting in an enhanced fluorescent emission intensity. In vivo detection of senescence is validated in mice bearing tumor xenografts treated with senescence-inducing chemotherapy. ; The authors thank the financial support from the Spanish Government (Projects MAT2015-64139-C4-1-R) and the Generalitat Valenciana (Project PROMETEOII/2014/047). B.L-T. is grateful to the Spanish Ministry of Economy for her PhD grant. I.G. thanks the Generalitat Valenciana for her PhD grant. A.B. thanks the Spanish Government for the financial support "Juan de la Cierva-Incorporacion". M.R. is grateful to "La Caixa" foundation for his PhD scholarship. Work in the M.S. group was funded by the CNIO and by grants from Spanish Ministry of Economy, the European Regional Development Fund (SAF project) the European Research Council (ERC Advanced Grant), and the Botin Foundation and Banco Santander (Santander Universities Global Division). D.M.-E. was funded by a "Ramon y Cajal" Programme Senior Grant and the "RETOS" National Programme for Research (MINECO), Cancer Research UK, and the CRUK Cambridge Centre Early Detection Programme. ; Lozano-Torres, B.; Galiana, I.; Rovira, M.; Garrido-García, EM.; Chaib, S.; Bernardos Bau, A.; Muñoz-Espín, D. (2017). An OFF-ON Two-Photon Fluorescent Probe for Tracking Cell Senescence in Vivo. Journal of the American Chemical Society. 139(26):8808-8811. https://doi.org/10.1021/jacs.7b04985 ; S ; 8808 ; 8811 ; 139 ; 26